| 000 | 02494cam a2200337 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223031645.0 | ||
| 008 | 170204s2016 ua h f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.24.05.M.Sc.2016.Ma.N | ||
| 100 | 0 | _aMahmoud Said Hamed Ali Amer | |
| 245 | 1 | 2 |
_aA novel transformation system, based on laser micro puncturing beam and gene coated gold particles in wheat / _cMahmoud Said Hamed Ali Amer ; Supervised Mona A. Aziz , Reda Abdelhalim |
| 246 | 1 | 5 | _aمنظومة مبتكرة للنقل الجينى مبنية على شعاع الليزر الميكرونى و جسيمات الذهب المطعمة بالجين فى القمح |
| 260 |
_aCairo : _bMahmoud Said Hamed Ali Amer , _c2016 |
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| 300 |
_a132 P. : _bfacsimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Applications in Environmental Photochemistry and Agriculture | ||
| 520 | _aWe report new transfection methods for intact plant cells that uses laser beam to introduce foreign gene-coated gold nanoparticles into wheat mature embryos cells (Triticum aestivum) through the cell wall and cell membrane. This homemade setup secures the transformation of as many as 30 mature embryo derived calli in less than one hour using second harmonic of Nd:YAG laser at 532nm(150mW) with two dimensional translation stages, a suitable computer program and a proper optical system. Seven days old calli were irradiated by a focused laser microbeam to puncture momentarily made self healing holes ({uF07E}0.5 æm) in the cell wall and membrane to allow uptake of foreign genes coated gold nanoparticles. The plant expression vector PBI-121 harboring the green fluorescent protein gene (gfp) under the control of 35-S promoter and nos terminator and the neomycin phosphotransferase-II gene (npt-II) as a selectable marker for the herbicide kanamycin sulfate resistance. Treated wheat tissue samples were then cultured for several weeks on the selection medium | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aLaser mediated gene transfer | |
| 653 | 4 | _aMature embryos | |
| 653 | 4 | _aWheat | |
| 700 | 0 |
_aMona Abdelaziz Mohamed , _eSupervisor |
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| 700 | 0 |
_aReda Elwany Abdelhalim , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aSamia _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c59663 _d59663 |
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