| 000 | 02569cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223031646.0 | ||
| 008 | 170206s2016 ua h f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.11.03.Ph.D.2016.Ma.M | ||
| 100 | 0 | _aMaha Mohamed Salah Eldin | |
| 245 | 1 | 0 |
_amiRNA 101-1 and miRNA 221 expressions and their polymorphisms as biomarkers for early diagnosis of hepatocellular carcinoma / _cMaha Mohamed Salah Eldin ; Supervised Olfat Gamil Shaker , George Nazih Bishara Morcos , Aisha Mahmoud Abdelaziz |
| 246 | 1 | 5 | _aالتعبير و التحور الجينى فى الميكرو أر إن إيه 1- 101 و221 كدلالات حيوية تنبؤية للتشخيص المبكر لسرطان الكبد |
| 260 |
_aCairo : _bMaha Mohamed Salah Eldin , _c2016 |
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| 300 |
_a160 P. : _bfacsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry | ||
| 520 | _aHepatocellular carcinoma (HCC) is the fifth most common malignant tumor with an increasing incidence. HCV is one of the major risk factors that lead to HCC development. MicroRNAs are conserved non-coding RNAs which regulate gene expression at the posttranscriptional level. They have been recently identified as important regulators that affect carcinogenesis. Of these miRNAs, are miR 221 and miR 101-1, which their aberrant expressions have been reported to play an important role in HCC. In this study, we aimed to investigate the association between miR-221 and miR-101-1 polymorphisms and their expressions and the early prediction of HCC in HCV infected patients. First, RNA was extracted from serum using (qiagen, valencia, CA, USA) then quantitative real-time PCR (qPCR) was done to estimate the expression levels of miRNA 221 and miRNA 101-1 in serum. To detect the genotyping of miR 221 and miR-101-1 related SNPs, DNA was extracted from whole blood using Qia amplification DNA extraction kit (qiagen, USA). Then, genotyping was performed using real-time PCR with TaqMan allelic discrimination assay Applied Biosystems, USA | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aHCC | |
| 653 | 4 | _aHCV | |
| 653 | 4 | _amiR 101-1 | |
| 700 | 0 |
_aAisha Mahmoud Abdelaziz , _eSupervisor |
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| 700 | 0 |
_aGeorge Nazih Bishara Morcos , _eSupervisor |
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| 700 | 0 |
_aOlfat Gamil Shaker , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aSamia _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c59704 _d59704 |
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