000			02903cam a2200337 a 4500
003			EG-GiCUC
005			20250223031719.0
008			170504s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.08.06.M.Sc.2016.Sh.A
100	0		_aShaimaa Mansour Abdelmonem Elmetwally
245	1	0	_aAssessment of bacterial virulence in chronic suppurative otitis media / _cShaimaa Mansour Abdelmonem ElMetwally ; Supervised Alaa Edin Mahmood Shawky Hosny , Ahmed Sherif Attia
246	1	5	_aتقييم المقدرة على إحداث المرض بالسلالات البكتيرية المسببة لالتهاب الأذن الوسطي الصديدي المزمن
260			_aCairo : _bShaimaa Mansour Abdelmonem Elmetwally , _c2016
300			_a100 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520			_aChronic suppurative otitis media (CSOM) is a recurrent or chronic infection of the ear, prevalent worldwide especially among the poor populations in developing countries, the infection is characterized by mucopurulent otorrhea through a perforated tympanic membrane and may end in hearing loss. Total of 80 bacterial strains were isolated from ear discharges of clinically diagnosed CSOM patients in two Egyptian hospitals. The isolates were identified using traditional methods along with the API® system. Percentages of different isolates obtained were about 58% Staphylococcus, 21% Pseudomonas, 20% Proteus and 1% Klebsiella. The isolates were screened for biofilm formation and antibiotic resistance, in addition to the complement resistance ability in Gram negative bacterial isolates. The probable correlations between the three studied factors were also investigated. Antibiotic susceptibility testing showed that about 54% of the isolates were multidrug resistant, the biofilm assay showed that about 20% of the isolates were biofilm forming, while the complement resistance existed in about 68% of the tested Gram negative bacteria. Methicillin resistant S. aureus (MRSA) represented the most common isolate of about (38%), accordingly the mecA gene and the dltB gene (biofilm formation-regulating gene) were screened using PCR technique, and this showed that 75% of the screened S. aureus isolates yielded positive mecA gene while 100% of the screened S. aureus isolates yielded dltB gene
530			_aIssued also as CD
653		4	_aChronic infection
653		4	_aChronic suppurative otitis media
653		4	_aOtitis media
700	0		_aAhmed Sherif Attia , _eSupervisor
700	0		_aAlaa Eldin Mahmood Shawky Hosny , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c60768 _d60768