000			02963cam a2200349 a 4500
003			EG-GiCUC
005			20250223031733.0
008			170614s2015 ua do f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.24.05.M.Sc.2015.Am.D
100	0		_aAmal Mohamed Ibrahim Osman
245	1	0	_aDiagnosis of honeybee diseases using PCR and laser spectroscopic techniques / _cAmal Mohamed Ibrahim Osman ; Supervised Mona Abdelaziz Mohamed , Emtithal Mohammed Abdelsamiea , Hamdy Taher Mohamed Abouelenain
246	1	5	_aو استطياف الليزر PCRتشخيص امراض نحل العسل باستخدام تقنيات ال
260			_aCairo : _bAmal Mohamed Ibrahim Osman , _c2015
300			_a152 P. : _bcharts , photographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Environmental Metrology Photochemistry and Agriculture
520			_aPaenibacillus larvae subsp. larvae, the causative pathogenic bacterium of virulent brood disease (American foulbrood, AFB). DNA extracted from P. l. larvae strain was subjected to PCR using specific primers of a partial sequence of 16S rRNA gene that closely related to the bacterium. Assayed bacterial strain produced a defined band of 700 bp in size. This test was done based on DNA {u2013} extraction of bacterial colonies and different larval stages(1-5 days, of age) from Carniolan (Apis mellifera carnica) honeybee and Italian (Apis mellifera ligustica), The results of PCR analysis showed that the (day1) of all honeybee races didn't amplify the pathogen fragment (700bp) and all larval stages from (day2- day5) generated PCR fragment of 700bp. Also, this defined fragment was detected in larvae, pupae, adult worker and virgin queen resulted from the grafting Italian honeybee. On the other hand, the physicochemical structure of genomic DNA of pathogenic bacterium P. l. larvae and the two honeybee races before (-ve control) &after infection with the pathogen were identified and characterized qualitatively and quantitatively using molecular laser Raman (MLRS) and infra-red (FTIR) spectroscopies for the first time. The results of spectroscopic analysis clearly revealed that the pathogen was more abundant in Italian race from the early infection stage (day1) to (day5) of age. than Carniolan which showed more resistant to pathogen infection of the (day2)
530			_aIssued also as CD
653		4	_aCarniolan and Italian honeybee races
653		4	_aP. l. larvae
653		4	_aPCR
700	0		_aEmtithal Mohammed Abdesamiea , _eSupervisor
700	0		_aHamdy Taher Mohamed Abouelenain , _eSupervisor
700	0		_aMona Abdelaziz Mohamed , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c61224 _d61224