000 02598cam a2200337 a 4500
003 EG-GiCUC
005 20250223031746.0
008 170722s2016 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.08.06.M.Sc.2016.Ne.B
100 0 _aNermeen Hosni Ali Elzairy
245 1 0 _aBiotechnological studies on microbial hemicellulases /
_cNermeen Hosni Ali Elzairy ; Supervised Abdelgawad M. Hashem , Mohamed A. Abdelnaby
246 1 5 _aدراسات بيوتكنولوجية على إنزيمات الهيميسليولاز الميكروبية
260 _aCairo :
_bNermeen Hosni Ali Elzairy ,
_c2016
300 _a160 P. :
_bcharts ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology
520 _aA survey of ten isolated fungal strains was carried out for the production of fungal xylanases. Aspergillus flavus isolate NERMEEN12 was the strain with the highest xylanase activity. Xylanase enzyme was produced by aspergillus flavus isolate utilizing different agricultural wastes rice straw, corn cobs, wheat bran, wheat straw and saw dust by solid state and submerged fermentation techniques. The highest xylanase production was obtained by solid state fermentation in the following order corn cobs >rice straw >wheat straw >wheat bran> saw dust. Enhancement of xylanase production was achieved by two statistically-based experimental designs to optimize the fermentation medium. The plackett-burman multifactorial design was first employed to investigate the effect of 19 factors on xylanase production. The most significant factors found to be, CaCl2.2H2O, CuSO4, CMC and NaCl. Further optimization was done using taguchi L9 (3^4) orthogonal array design, by which the effect of four factors in three levels, could be tested. The taguchi design showed that the maximum yield of xylanase was produced when CaCl2.2H2O, CuSO4, CMC and NaCl were present in the fermentation medium in concentrations (g/l): 1, 0.2, 10 and 10 respectively. Under optimal conditions enzyme production was increased by 2.4-fold compared to the non optimized medium
530 _aIssued also as CD
653 4 _aAspergillus flavus isolate
653 4 _aMultifactorial design
653 4 _aXylanase
700 0 _aAbdelgawad Mohamed Hashem ,
_eSupervisor
700 0 _aMohamed Ahmed Abdelnaby ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c61637
_d61637