000			02819cam a2200325 a 4500
003			EG-GiCUC
008			170806s2017 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.26.Ph.D.2017.En.I
100	0		_aEnas Aly Elsaftawy
245	1	0	_aImpact of different therapeutic approaches in hydatid cyst vitality and patients{u2019} morbidity / _cEnas Aly Elsaftawy ; Supervised Amany Ahmed Abdelaal , Manal Abdelmegeed Badawi , Samar Sayed Attia
246	1	5	_aتأثير طرق علاجية مختلفة علي حيوية اكياس العطش و حالة المرضي المصابين بها
260			_aCairo : _bEnas Aly Elsaftawy , _c2017
300			_a194 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Parasitology
520			_aThe aim of this scientific work was to assess the impact of different therapeutic approaches, including medical and surgical interventions on hepatic hydatid cyst vitality and patients' morbidity. In the current study, 40 hydatid cyst fluid samples from the included cases were processed and examined by different methods to determine viability status. 1% eosin exclusion method was used to initially test for protoscolices (PSs) viability, followed by specific fluorescent dyes to explore internal cellular kinetics in both PSs and germinal epithelium (anti-mitochondrial and anti-prohibitin antibodies). In addition to nucleic acid stains (DAPI and PI), plus morphocyto-micrometeric analysis, which were done for the cystic components, using image analyser. According to the viability reported by different means, patients received only one dose of chemotherapy recorded significantly higher viability in PSs (55-85%), followed by those treated by single PAIR plus single dose of medical treatment (25 to 45%), indicating failure of these therapeutic approaches to completely eradicate the powerful parasitic stage. While the least viability was reported, when PIAR accompanied by repeated doses of medical treatment were used (0 to 10%). This study recommended the reconsideration of the protocols, currently applied to treat cystic hepatic echinococcosis. This in order to minimize patients' suffers and recurrence from the still living PSs or germinal epithelium, however in a stationary phase, ready in the proper time to cause threatening morbidity
530			_aIssued also as CD
653		4	_aCyst fluid
653		4	_aHydatid
653		4	_aViability
700	0		_aAmany Ahmed Abdelaal , _eSupervisor
700	0		_aManal Abdelmegeed Badawi , _eSupervisor
700	0		_aSamar Sayed Attia , _eSupervisor
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c61828 _d61828