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008 170911s2016 ua dho f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.05.M.Sc.2016.Ma.P
100 0 _aMarwa Moustafa Abdelaliem
245 1 0 _aPotential use of some microorganisms for suppression of brown rot disease in potato (solanum tubersum) caused by ralstonia solanacearum /
_cMarwa Moustafa Abdelaliem ; Supervised Zeinat Kamel Mohamed , Nevein Anwar Shehata Messiha
246 1 5 _aRalstonia solanacearum إمكانية بعض الكائنات الدقيقة على تثبيط مرض العفن البنى فى البطاطس المتسبب عن
260 _aCairo :
_bMarwa Moustafa Abdelaliem ,
_c2016
300 _a149 P. :
_bcharts , facsimiles , photographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany-Microbiology
520 _aPotato brown rot caused by ralstonia solanacearumrace 3 biovar 2 (phylovar II, sequevar 1) is one of the most devastating bacterial disease and have a negative impact in potato production and exportation. It is a quarantine disease with zero tolerance and exportation is restricted to pest free areas (PFAs) in the newly reclaimed areas. This thesis aimed to find new safe microorganism which can be used as potential biocontrol agent against potato brown rot. screening of antagonistic biocontrol agents was made from potato soil and rhizosphere following different fertilization regimes, five antagonistic bacterial isolates showed similarity with P. fluorescens, bacillus megaterium, azotobacter chroococcum and delftia sp. On the other hand, ten antagonistic actinomycetes strains were isolated mostly from sandy soils and showed antagonistic potential against R. solanacearum. Four Actinomycetes potential antagonists were identified using cultural characteristics, electron microscope scan and 16S rDNA. The four isolates showed similarity with S. griseoincarnatus, S. deserti, S. anulatus and S. thermocarboxydus. Some biochemical activity of these isolates were addressed in vitro. S. thermocarboxydus showed the highest proteolytic activity followed by S. anulatus and S. deserti showed minor activity. Also, streptomyces deserti gave the highest ammonia production followed by streptomyces thermocarboxydus and finally Streptomyces anulatus while S. griseoincarnatus showed no ammonia production. The same isolates showed similar phosphate solubilizing activity
530 _aIssued also as CD
653 4 _aBiocontrol agents
653 4 _aPotato brown rot
653 4 _aR. solanacearumrace
700 0 _aNevein Anwar Shehata Messiha ,
_eSupervisor
700 0 _aZeinat Kamel Mohamed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c62253
_d62253