000			03102cam a2200325 a 4500
003			EG-GiCUC
008			170911s2016 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.25.M.Sc.2016.Om.P
100	0		_aOmar Ahmed Khaled Taha Ahmed Eldahshoury
245	1	0	_aPunica granatum extract as a regulator of low-density lipoprotein receptor (LDLR) and HMG CoA reductase gene expression in hepG2-cell line / _cOmar Ahmed Khaled Taha Ahmed Eldahshoury ; Supervised Magda Ahmed Abdallah , Alaa Ahmed Barakat , Heba Mohamed Kamal
246	1	5	_aفى خلايا الكبد السرطانية (LDLR) و مستقبلات البروتينات الدهنية منخفضة الكثافة (HMG CoA reductase) مستخلص الرمان كمنظم طبيعى للتعبير الجينى لانزيم مختزل الهيدروميثيل جلوتاريل كو - أيه
260			_aCairo : _bOmar Ahmed Khaled Taha Ahmed Eldahshoury , _c2016
300			_a119 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology
520			_aObjective: Pomegranate (punica granatum L., Lythraceae) peel has concentrated amounts of lipid-lowering elements that demonstrated, in various hoary and recent studies, their effects against obesity and hyperlipidemia, involving elevated rates of lipid and lipoprotein levels in blood and increases risking of cardiovascular diseases and other diseases. We aim to study expression modulation of genes involved in lipid metabolism by impact of standardized pomegranate peel extract (PPE) in a comprehensive research on human liver cells and experimental mice. Methods: Initially, after extraction process, reversed-phase HPLC was used to determine amounts of ellagic acid as a standard phytochemical. And then, using reverse-transcription real-time PCR, an in-vitro study harnessing HepG2 cell-line was conducted to determine the hyperlipidemia-related gene expression profiles and cytotoxic effects upon treatment with PPE. In another complementary in-vivo study, male C57BL/6J mice were fed a high-fat diet (HFD) or an HFD supplemented with PPE for 14 days to define expression of lipid-metabolism related genes that control obesity. Correspondingly, DNA fragmentation effect and fatty liver proportions were also estimated after treatment. Results: Higher mRNA expression of LDL receptor (LDL-R) and down-regulation of sterol regulatory element-binding protein (SREBF-2), (SRBEP-1c), Fatty acid synthase (FAS) and 3-hydroxy-3- methylglutaryl-CoA reductase (HMGCR) upon PPE treatment in HepG2 cell-line were significantly recorded analysis.
530			_aIssued also as CD
653		4	_aHyperlipidemia
653		4	_aPeel extract
653		4	_aPunica granatum L.
700	0		_aAlaa Ahmed Barakat , _eSupervisor
700	0		_aHeba Mohamed Kamal , _eSupervisor
700	0		_aMagda Ahmed Abdallah , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c62254 _d62254