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003 EG-GiCUC
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008 170912s2016 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.07.Ph.D.2016.Di.D
100 0 _aDina Samir Abdelhady
245 1 4 _aThe differentiation potential of human cord blood- derived mesenchymal stem cells into functional hepatocyte-like cells on nanofibrous scaffold /
_cDina Samir Abdelhady ; Supervised Taghreed Mohamed Gaafar , Zeinab Abou Bakr Demerdash , Nermine Magdi Riad
246 1 5 _aقدرة الخلايا الجذعية الميزنشية المستخلصة من دم الحبل السري المزروعة علي انسجة النانو (سمافولد) علي التمايز الي خلايا كبدية فعالة
260 _aCairo :
_bDina Samir Abdelhady ,
_c2016
300 _a204 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine- Department of Clinical and Chemical Pathology
520 _aBackground: Mesenchymal stem cell (MSC) based liver tissue engineering on nano-fibrous scaffold holds great promise for cell-based therapy in end- stage liver failure. Scaffolds are three-dimensional (3D) matrices that provide the initial structural support for cells to attach, proliferate, and differentiate, facilitating the formation of an extracellular matrix (ECM). Aim of the Work: In this study we examined the differentiation potential of MSCs into hepatocytes in two- and three-dimensional (2D and 3D) culture systems to improve the in vitro-hepatic differentiation of MSCs to be used in tissue-based therapies in patients with chronic liver diseases. Materials and Methods: MSCs were isolated from human umbilical cord blood (UCB). Hepatogenic differentiation was induced on 2D and 3D culture system and hepatogenic function was evaluated by immunocytochemistry, Periodic acid Schiff staining (PAS), enzyme linked immunosorbent assay (ELISA), and gene expression analysis. Differentiated cells were injected intravenously (IV) into murine model of carbon tetra chloride (CCL4) induced liver fibrosis. Animal models were divided into 3 groups, including pathological control group injected with CCL4, CCL4-induced liver fibrotic group treated with 2D differentiated cells, and CCL4-induced liver fibrotic group treated with 3D differentiated cells. Livers were stained with anti-human alpha-feto protein (AFP) and albumin immunoperoxidase staining. Sera were collected and tested for Alanine transaminase (ALT), aspartate aminotransferase (AST), and albumin
530 _aIssued also as CD
653 4 _aMesenchymal stem cells
653 4 _aMesenchymal stem cells (MSCs)
653 4 _aNanofibrous scaffold
700 0 _aNermine Magdi Riad ,
_eSupervisor
700 0 _aTaghreed Mohamed Gaafar ,
_eSupervisor
700 0 _aZeinab Abou Bakr Demerdash ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c62302
_d62302