| 000 | 02839cam a2200349 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223031820.0 | ||
| 008 | 171004s2017 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.12.02.Ph.D.2017.Gh.E | ||
| 100 | 0 | _aGhada Hamdi Elsayed Mohamed | |
| 245 | 1 | 0 |
_aEffect of some newly synthesized steroid derivatives on apoptosis of breast cancer cells / _cGhada Hamdi Elsayed Mohamed ; Supervised Rafat Milad Mohareb , Mervat Sayed Mohamed , Gamal Abdelmegeed Abdelghany |
| 246 | 1 | 5 | _aتأثير بعض المشتقات الستيرويدية الجديدة المشييدة على موت الخلايا المبرمج فى سرطان الثدى |
| 260 |
_aCairo : _bGhada Hamdi Elsayed Mohamed , _c2017 |
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| 300 |
_a97 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry | ||
| 520 | _aAnticancer agents consisting of hybrid molecules are used to improve effectiveness and diminish drug resistance. The current study aimed to introduce newly synthesized hetero-steroids of promising anticancer effects. Besides, the pro-apoptotic effects of new compounds were investigated extensively. Several pyrimidino-, triazolopyrimidino-, pyridazino-, and curcumin- steroid derivatives were synthesized, elucidated and confirmed using the spectral and analytical data. The synthesized hetero-steroids, compounds 9, 10, 11, 12, 13, 14, 15, 18, 20, 21, 22 and 24, were tested for their cytotoxic effects versus human breast cancer cells (MCF-7) using neutral red supravital dye uptake assay. Compound 24 (IC50 = 18 æM) showed more inhibitory influence on MCF-7 growth. Using QRT-PCR (Quantitative real time-polymerase chain reaction), CCND1, Survivin, BCL-2, CDC2, P21 and P53, genes expression levels were investigated. The study results disclose that compounds 4, 7, 18, 24 knocked down the expression levels of CCND1, Survivin, BCL-2 and CDC2. However, P21 and P53 were up- regulated by compounds 21, 22. This study introduced promising pro-apoptotic anticancer agents acting through the modulation of key regulators of apoptosis and cell cycle genes. The current study revealed the most promising compounds 7, 18, 21 down-regulated miR-34a, miR-98 and miR-214 expression levels and decreased upon drug resistance response. | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aBreast cancer | |
| 653 | 4 | _aHeterocycles | |
| 653 | 4 | _aSteroids | |
| 700 | 0 |
_aGamal Abdelmegeed Abdelghany , _eSupervisor |
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| 700 | 0 |
_aMervat Sayed Mohamed , _eSupervisor |
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| 700 | 0 |
_aRafat Milad Mohareb , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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| 905 |
_aShimaa _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c62752 _d62752 |
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