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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.16.03.Ph.D.2017.Da.E
100 0 _aDalia Abdellatief Ibrahim Kishk
245 1 0 _aEffect of some chemical mutagens on improving the micropropagated Banana plants to tolerate drought and salinity stresses /
_cDalia Abdellatief Ibrahim Kishk ; Supervised Elsayed I. Gaber , Adel A. Abulsoad , Hattem M. Elshabrawi
246 1 5 _aبعض المطفرات الكيميائية على تحسين تحمل نباتات الموز المكثرة معملياً للإجهاد المائي والملحي
260 _aCairo :
_bDalia Abdellatief Ibrahim Kishk ,
_c2017
300 _a213 P. :
_bcharts , facsmiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Institute of African Research and Studies - Department of Natural Resources
520 _aThis study was carried out at the Tissue Culture Laboratory of the Horticulture Res. Inst. (HRI), Agricultural Research Center (ARC) and the Plant Biotechnology Dept., National Research Center (NRC) during the periods from 2012 till 2015. The aim of this study was to induce a tolerance mutant in banana plants for salt and drought stresses by using different chemical mutagenesis diethyl sulphate (DES) and sodium azide (NaN₃) and concentrations (100 oM for DES and 2 oM for NaN₃ expressed as low, 200 oM for DES and 4 oM for NaN₃ expressed as medium and 400 oM for DES and 8 oM for NaN₃ expressed as high) as well as soaking durations (10, 20 and 30 min) to increase its ability to tolerate salinity (500, 1000 and 1500 ppm) and drought stresses (PEG at 8, 16 and 32 gl⁻¹). Salinity and drought experiments were carried out during rooting stage and evaluated until the end of acclimatization stage. During multiplication stage, the results showed that NaN₃ was more effective in increasing number of shoots/plantlet but DES increased number of leaves and shoot length. High concentration for 30 min increased number of shoots and leaves to the highest values. Interaction between mutagen and concentrations plus duration revealed that DES concentrations produced the highest values except for number of shoots/plantlet which was more effective by using NaN₃ at 8 oM for 30 min. There were a great variance regarding the effect of mutagens, concentrations plus duration and studied stress (salinity and drought), individually and double interaction between these three factors during rooting and acclimatization stages. Treble interaction investigated that, DES at 100, 200 and 400 oM (to some extent) for different durations (10 to 30 min) could be used to reduce the negative effects of salinity (up to 1500 ppm) and drought (up to 32 g/l) during both rooting and acclimatization stages, with little exceptions toward NaN₃
530 _aIssued also as CD
653 4 _aBanana Grand Nain cv
653 4 _aDES
653 4 _aDiethyl sulphate
700 0 _aAdel A. Abulsoad ,
_eSupervisor
700 0 _aElsayed I. Gaber ,
_eSupervisor
700 0 _aHattem M. Elshabrawi ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c63574
_d63574