| 000 | 03224cam a2200337 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223031855.0 | ||
| 008 | 171211s2016 ua do f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.09.12.M.Sc.2016.No.C | ||
| 100 | 0 | _aNoha Ahmed Elsherif | |
| 245 | 1 | 0 |
_aCharacterization of surface markers of salivary gland stem cells versus periodontal ligament stem cells in albino rats : _bIn vitro study / _cNoha Ahmed Elsherif ; Supervised Ratiba Ghali Abdelmalek , Marwa Sameer Moussa |
| 246 | 1 | 5 |
_aتوصيف علامات سطح الخلايا الجذعية للغدد اللعابية مقابل الخلايا الجذعية لرباط اللثة فى الفئران البيضاء : _bدراسة معملية |
| 260 |
_aCairo : _bNoha Ahmed Elsherif , _c2016 |
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| 300 |
_a97 P. : _bcharts , photographs ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral Biology | ||
| 520 | _aObjective: The aim of the present study is to isolate and characterize salivary gland stem cells and periodontal stem cells from adult albino rat. A comparison was carried out between their surface markers expression utilizing surface markers for flow cytometry. The odontogenic differentiation of salivary gland stem cells and periodontal ligament stem cells was assessed. Material and methods: Samples were collected from the two sources. PDLSCs and SGSCs were treated with collagenase before centrifugation to allow release of the cells. Cells were cultured in complete culture medium for 12-14 days. After reaching confluence, the isolated cells were characterized by flow cytometry using CD133 and C-Kit. The cells were induced for odontogenic differentiation by placing the cells in odontogenic induction media for 21 days. Odontogenic differentiation was evaluated by Alizarin Red stain and by the expression of dentine sialophosphoprotein (DSPP) which was assessed by RT-PCR. Results: The results showed that successful isolation of stem cells from periodontal ligament and salivary gland was achieved. Cells were successfully sub-cultured and expanded up to passage three. A flow cytometric analysis was done after stem cells isolation. PDLSCs were negatively expressed CD133 and C-Kit while SGSCs showed moderately positive expression of CD133 and C-KIT. The results showed that PDLSCs and SGSCs induced by odontogenic medium were stained by alizarin red at days 14 and 21. RT-PCR results indicated that all cultured cells efficiently differentiate into dentin forming cells and expressed specific DSPP gene which was expressed higher by PDLSCs than SGSCs in days 14 and 21. Conclusion: It can be concluded that PDLSCs show higher differentiation into odontogenic linage than SGSCs | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aAlbino rats | |
| 653 | 4 | _aSalivary gland stem cells | |
| 653 | 4 | _aSurface markers | |
| 700 | 0 |
_aMarwa Sameer Moussa , _eSupervisor |
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| 700 | 0 |
_aRatiba Ghali Abdelmalek , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aSamia _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c63951 _d63951 |
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