000			03003cam a2200325 a 4500
003			EG-GiCUC
008			171223s2017 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.07.04.Ph.D.2017.Ha.M
100	0		_aHayam Fouad Ibrahim Sayed
245	1	0	_aMolecular genetic studies on some drought and salinity tolerance genes in bread wheat / _cHayam Fouad Ibrahim Sayed ; Supervised Abdelkader Morsy Abdelsamad , Osama Konsowa Ahmed , Sabry Abdalla Mohammed Khatab
246	1	5	_aدراسات وراثية جزيئية على بعض جينات تحمل الجفاف والملوحه فى قمح الخبز
260			_aCairo : _bHayam Fouad Ibrahim Sayed , _c2017
300			_a171 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Agriculture Biochemistry
520			_aThe aims of this study were identifying of some wheat varieties and double haploid response to drought and salinity stress to determine and isolation of tolerant genes and select a gene for cloning it in tobacco plant. The behavior of seven wheat (tritium aestivum) genotypes under salinity and drought stress was studied. The genotypes used were double haploids (DH1, DH2, DH3 and DH4) and local verities (Sakha93, Sids1 and Gemmiza9) and relative water content (RWC) was estimated for the varieties and DH under drought stress 30% polyethylene glycol 6000, after 72 hours of treatment, The genotypes DH4 had the higher RWC (91.93%) while Sids1 had the lowest RWC of 68.23%. under water stress 30% PEG-6000. While, at salinity level 2.5% NaCl, DH3 displayed the highest relative water content (92.71%) and Gimmeza9 had the lowest RWC 75.88%. Using primers and polymerase chain reaction (PCR) technique, the genes WZY2, WUB3, ZFP22, TaOF1b, STRP and Di19a were detected in all genotypes and gave fragments with MW. 194,234,182,204,228 and 747bp respectively, with variance expression between different genotypes. Three genes were selected for study the expression of whole sequence genes. DH4 the highest RWC genotype under drought stress and DH3 was had the highest RWC genotype under salinity stress at different times 0, 3, 6,12,24,48 and 72h, in leaves and roots, The wzy2 gene appeared 474bp which varied in expression between leaves and roots, while STRP with 879bp appeared in DH4 in root only at 48h, however in DH3 under salinity stress no gene expression was appeared. On the other hand, Di19a with 747bp appeared in DH3 at 24 and 48h under salinity stress
530			_aIssued also as CD
653		4	_aDrought and salinity stress
653		4	_aTritium aestivum
653		4	_aWheat
700	0		_aAbdelkader Morsy Abdelsamad , _eSupervisor
700	0		_aOsama Konsowa Ahmed , _eSupervisor
700	0		_aSabry Abdalla Mohammed Khatab , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c64173 _d64173