000			02869cam a2200349 a 4500
003			EG-GiCUC
005			20250223031902.0
008			171223s2017 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.07.04.M.Sc.2017.Is.B
100	0		_aIsmail Guda Said Ismail
245	1	0	_aBiochemical and physiological studies on the production of some pharmaceutical compounds / _cIsmail Guda Said Ismail ; Supervised Emam Abdelmobdy Abdelrahim , Magdy Abdelaleem Mohamed Shallan , Kamal Yousef Ibraheim Eshahed
246	1	5	_aدراسات كيميائية حيوية وفسيولوجية على إنتاج بعض المركبات الدوائية
260			_aCairo : _bIsmail Guda Said Ismail , _c2017
300			_a150 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Agriculture- Department of Agriculture Biochemistry
520			_aThe main objective of the present study is to improve clavulanic acid (CA) production using UV-mutagenesis on Streptomyces sp. NRC 77. From the screened mutants, 120A3 mutant isolate was selected as a promising one .The biochemical, morphological, physiological properties of 120A3 mutant showed culture characteristics similar to Streptomyces sp. Phylogenetic analysis of the 16S rDNA gene sequence showed that this strain has a similarity of (99 %) to Streptomyces sp. T2-7, therefore it was suggested as Streptomyces sp. MU-NRC 77 and its nucleotide sequence was deposited in Gen Bank under the accession No. KT953342. Improvement of CA yield by 572.49 mg/l was obtained from fermentation medium and culture condition optimization in compared with 130.69 mg/l for wild strain. Further optimized by the addition of H2O2 and activated plant charcoal (1%) to the production medium increased CA yield strongly to reach 646.12 and 682.94 mg/l respectively by production incresement 5.2 fold more than that obtained before adding. Production of CA by the new selected Streptomyces mutated isolate using bioreactor 7L under optimized medium and conditions (agitation speed effect) afforded the highest CA yield 719.75 mg/l at 600 rpm by production incresement 5.5 fold in comparison with the wild strain, then extraction, purification of the CA from fermentation broth and chemical elucidation was carried out using different spectroscopic analysis
530			_aIssued also as CD
653		4	_aClavulanic acid
653		4	_aPhylogenic analysis
653		4	_aUV-mutagenesis
700	0		_aEmam Abdelmobdy Abdelrahim , _eSupervisor
700	0		_aKamal Yousef Ibraheim Eshahed , _eSupervisor
700	0		_aMagdy Abdelaleem Mohamed Shallan , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c64191 _d64191