000 03181cam a2200349 a 4500
003 EG-GiCUC
005 20250223031903.0
008 171224s2016 ua o f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.12.21.M.Sc.2016.Sa.A
100 0 _aSara Mohamed Abdelkhalek Abdelrazek
245 1 0 _aAssessment of the trans-differentiation potential of bone marrow and umbilical cord blood stem cells in generating insulin producing cells /
_cSara Mohamed Abdelkhalek Abdelrazek ; Supervised Mahmoud Ahmed Amer , Amira Mohamed Raafat , Abeer Ahmed Bahnassy
246 1 5 _a{uFE97}{uFED8}{uFEF4}{uFEF4}{uFEE2} إ{uئإإ٣}{uئإؤأ}{uئإ٨إ}{uئإإ٧}{uئإئ٤}{uئإ٩٤} {uئإ٩٧}{uئإإ٤}{uئإ٨إ}{uئإئ٣}{uئإآ٠} ا{uئإؤئ}{uئإء٨}{uئإئأ}{uئإئ٣}{uئإ٨إ} {uئإإ٣}{uئإإ٦} {uئإإ٧}{uئإء٨}{uئإ٨إ}ع ا{uئإؤئ}{uئإأأ}{uئإأ٨}{uئإإ٢} و ا{uئإؤئ}{uئإء٨}{uئإئأ}{uئإئ٣}{uئإ٨إ} ا{uئإؤئ}{uئإء٠}{uئإءأ}{uئإأآ}{uئإئ٤}{uئإ٩٤} {uئإؤئ}{uئإءء}م ا{uئإؤئ}{uئإء٤}{uئإ٩٢}{uئإؤإ} ا{uئإؤئ}{uئإآ٤}{uئإءإ}ى فى {uئإ٩٧}{uئإإإ}{uئإؤئ}{uئإئ٤}{uئإءء} {uئإء٧}{uئإئأ}{uئإئ٣}{uئإ٨إ} إ{uئإإ٧}{uئإ٩٨}{uئإ٨إ}ج ا{uئإئ٧}{uئإإ٧}{uئإآ٤}{uئإإإ}{uئإؤئ}{uئإئ٤}{uئإإ٦}
260 _aCairo :
_bSara Mohamed Abdelkhalek Abdelrazek ,
_c2016
300 _a137 P. :
_bphotographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology
520 _aThis study was carried out to assess the feasibility of generating insulin-producing cells from progenitor cells obtained from the bone marrow (BM) and cord blood (CB) and compare the trans-differentiation potential of the two studied sources (BM versus CB cells). I- In this study, 20 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in-vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology insulin-producing cells appeared. II- Twenty samples of rat bone marrow were obtained; isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology, and became insulin-producing cells appeared. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of GAPDH, Insulin genes were assessed using RT-PCR technique
530 _aIssued also as CD
653 4 _aBone marrow
653 4 _aInsulin producing cells
653 4 _aUmbilical cord blood stem cells
700 0 _aAbeer Ahmed Bahnassy ,
_eSupervisor
700 0 _aAmira Mohamed Raafat ,
_eSupervisor
700 0 _aMahmoud Ahmed Amer ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c64220
_d64220