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005 | 20250223031903.0 | ||
008 | 171224s2016 ua o f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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041 | 0 | _aeng | |
049 | _aDeposite | ||
097 | _aM.Sc | ||
099 | _aCai01.12.21.M.Sc.2016.Sa.A | ||
100 | 0 | _aSara Mohamed Abdelkhalek Abdelrazek | |
245 | 1 | 0 |
_aAssessment of the trans-differentiation potential of bone marrow and umbilical cord blood stem cells in generating insulin producing cells / _cSara Mohamed Abdelkhalek Abdelrazek ; Supervised Mahmoud Ahmed Amer , Amira Mohamed Raafat , Abeer Ahmed Bahnassy |
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_aCairo : _bSara Mohamed Abdelkhalek Abdelrazek , _c2016 |
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_a137 P. : _bphotographs ; _c25cm |
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502 | _aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology | ||
520 | _aThis study was carried out to assess the feasibility of generating insulin-producing cells from progenitor cells obtained from the bone marrow (BM) and cord blood (CB) and compare the trans-differentiation potential of the two studied sources (BM versus CB cells). I- In this study, 20 samples of umbilical cord blood were obtained, isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in-vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology insulin-producing cells appeared. II- Twenty samples of rat bone marrow were obtained; isolation of mesenchymal stem cells (MSCs) was done. In vitro expansion of MSCs was performed. MSCs were propagated in vitro, the insulin-producing cells differentiation medium was added. The cells showed a change in their morphology, and became insulin-producing cells appeared. III. RNA extraction was performed; RNA was transformed to (cDNA) and expression of GAPDH, Insulin genes were assessed using RT-PCR technique | ||
530 | _aIssued also as CD | ||
653 | 4 | _aBone marrow | |
653 | 4 | _aInsulin producing cells | |
653 | 4 | _aUmbilical cord blood stem cells | |
700 | 0 |
_aAbeer Ahmed Bahnassy , _eSupervisor |
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700 | 0 |
_aAmira Mohamed Raafat , _eSupervisor |
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700 | 0 |
_aMahmoud Ahmed Amer , _eSupervisor |
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856 | _uhttp://172.23.153.220/th.pdf | ||
905 |
_aNazla _eRevisor |
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905 |
_aSamia _eCataloger |
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_2ddc _cTH |
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_c64220 _d64220 |