000			03345cam a2200349 a 4500
003			EG-GiCUC
005			20250223031920.0
008			180130s2017 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.18.M.Sc.2017.Ra.O
100	0		_aRadwa Ashour Mohamed
245	1	0	_aOccurrence of histamine producing bacteria in fish and its public health importance / _cRadwa Ashour Mohamed ; Supervised Maha Ahmed Sabry , Eman Aly Elghareeb Hamza , Hayam Abdelaal Mansour
246	1	5	_aتواجد الميكروبات المنتجة للهستامين فى الأسماك وأهميتها الصحية
260			_aCairo : _bRadwa Ashour Mohamed , _c2017
300			_a109 P. : _bcharts ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Zoonoses
520			_aHistamine fish poisoning becomes highly concern not only in public health but also economic aspect. Therefore, this study was attempted to detect histamine producing bacteria (HPB) in fish and fish handlers, as well as in canned tuna. Screening of 207 fish samples using Modified Niven{u2019}s medium (MNM), evidenced 108 isolated colonies were HPB, and significantly higher proportion wasin fresh sardine (95.7%), followed by frozen mackerel (53 %), frozen sardine (36.4 %) and canned tuna (14 %). Among those, only 88 isolates showed HPB based on plating on Violet Red Bile Glucose Agar (VRBG) agar, were 83 isolates identified as Enterobacteriacae, while only 5 isolates were identified as non-Enterobacteriacae. The most common isolates were K. pneumonia, E. aerogenes, Enterobacter cloacae and C. freundii following the biochemical identification using API20E. Out of 83 HP Enterobacteriacea strains from fish muscles 22 isolates (26.5%) were positive for hdc gene using conventional PCR, likewise, Citrobacter braakii evidenced hdc gene. While out of 5 non- Enterobacteriacea strains only one isolate was positive for hdc gene. The measuring of histamine level in some selected positive fish muscles using High-Performance Liquid Chromatography (HPLC) showed that the level of histamine in sardine muscles is significantly higher than that in mackerel muscles. On the other side, out of 20 hand swabs from fish handlers, 19 isolates of HPB were identified as Enterobacteriacea, and classified as K. pneumonia,C. freundii, E. cloacae and K. oxytoca, surprisingly, only one isolate was positive for hdc gene. A strong correlation was noticed between the occurrence of certain species of HP-Enterobacteriaceae in both fish and fish handlers at same localities. Phylogenetic analysis of hdc gene of different bacterial species,highlight the probability that the gram negative hdc gene might get transferred within the same and between the different genus of bacteria, this is alarming as they can be potential hazard to humans
530			_aIssued also as CD
653		4	_aCanned tuna
653		4	_aMackerel
653		4	_aSardine
700	0		_aEman Aly Elghareeb Hamza , _eSupervisor
700	0		_aHayam Abdelaal Mansour , _eSupervisor
700	0		_aMaha Ahmed Sabry , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c64742 _d64742