000 02752cam a2200349 a 4500
003 EG-GiCUC
005 20250223031921.0
008 180131s2017 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.07.Ph.D.2017.Re.C
100 0 _aReem Mohamed Ahmed Embaby
245 1 0 _aClinical role of micro-rnas expression in acute myeloid leukemia :
_bmiR-155, miR-424 deregulation, correlation With NPM1 /
_cReem Mohamed Ahmed Embaby ; Supervised Laila Abdelrahman Hegazy , Hanan Ramadan Nassar , Nohair Soliman
246 1 5 _aالدور الاكلينيكي للتعبير عن الحامض النووي الريبي الدقيق بنوعيه 155 و 424 في حالات سرطان الدم الميلودي الحاد وعلاقته مع جين النيكليوفوزمين
260 _aCairo :
_bReem Mohamed Ahmed Embaby ,
_c2017
300 _a149 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
520 _aAML is a highly heterogeneous disease characterized by failure of myeloid precursor cells to undergo terminal differentiation from proliferating precursor cells into mature blood cells. MicroRNAs (miRNAs) are small, non-coding RNAs control the expression of a number of genes involved in commitment and differentiation of hematopoietic stem cells and tumorigenesis. The study aimed to clarify role of miR-155,mir-424,in pathogenesis ,prognosis of AML and correlation of both miRNAs with NPM1 gene .The present study done on 40 adult newly diagnosed AML cases that were taken from National Cancer Inistitute & medical oncology unit Kasr Aini School of Medicine, Cairo University and 40 sex & age matched controls. A quantitative reverse-transcription PCR (qRT-PCR) assay was done to relatively quantify miR-424,miR-155. (RT-PCR) assay was done to assess NPM1 mutation and correlate it with both micro-RNAs.There was highly statistically significant difference in miR-424 between both patients and controls (p=0.0001),miR 155 was a bad predictor for AML cases. No correlation was found between miR -424, miR-155 and NPM1 .So that deregulated expression of both miRNAs does not depend on NPM1 to exhibit its role in leukemogenesis
530 _aIssued also as CD
653 4 _aAcute Myeloid Leukemia
653 4 _amiR-155
653 4 _amiR-424
700 0 _aHanan Ramadan Nassar ,
_eSupervisor
700 0 _aLaila Abdelrahman Hegazy ,
_eSupervisor
700 0 _aNohair Soliman ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c64763
_d64763