| 000 | 02642cam a2200325 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 008 | 180310s2016 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.12.21.Ph.D.2016.Am.E | ||
| 100 | 0 | _aAmal Mahfouz Abdelreheim | |
| 245 | 1 | 0 |
_aEffective diagnosis of human Toxoplasmosis using superparamagnetic nanoparticles by ELISA technique / _cAmal Mahfouz Abdelreheim ; Supervised Azza Mohamed Elamir , Noha Ahmed Mahana , Alyaa Ahmed Farid |
| 246 | 1 | 5 | _aالتشخيص الفعال لمرض التكسوبلازما في الإنسان بإستخدام الجزيئات النانونيه لأشباه المغناطيس الفائقة بتقنية الأليزا |
| 260 |
_aCairo : _bAmal Mahfouz Abdelreheim , _c2016 |
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| 300 |
_a112 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology | ||
| 520 | _aParasitic diseases have a great impact in human health. The gold standard for the diagnosis of the majority of parasitic infections is still conventional microscopy, which presents important limitations in terms of sensitivity and specificity and commonly requires highly trained technicians. More accurate technique, gold nanoparticles (AuNPs) conjugated with anti-Toxoplasma gondii (T. gondii) polyclonal antibody (pAb) was evaluated to increase the sensitivity and specificity of antigen detection assay (sandwich-ELISA and dot-ELISA) for diagnosing human T. gondii infection. In the present study, T. gondii SAG2 antigen was purified (37 KDa) and anti-SAG2 IgG pAb was prepared in rabbits. T. gondii SAG2 antigen was early detected in control and patient sera using nanogold sandwich and nanogold dot-ELISA. Two different nanogold ELISA showed a higher significant sensitivity (94% and 98%) and specificity (96% and 96%) in comparison to normal sandwich and dot-ELISA which showed 92% and 96% sensitivity and 90% and 94% specificity, respectively. From all recorded data and with consideration to sandwich and dot-ELISA assays, nanogold-sandwich and nanogold dot-ELISA assay have a promising and important value as an applicable, fast and accurate diagnostic technique for toxoplasmosis | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aDiagnosis | |
| 653 | 4 | _aSandwish ELISA | |
| 653 | 4 | _aT. gondii | |
| 700 | 0 |
_aAlyaa Ahmed Farid , _eSupervisor |
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| 700 | 0 |
_aAzza Mohamed Elamir , _eSupervisor |
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| 700 | 0 |
_aNoha Ahmed Mahana , _eSupervisor |
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| 905 |
_aNazla _eRevisor |
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| 905 |
_aShimaa _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c65345 _d65345 |
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