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003 EG-GiCUC
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008 180317s2017 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.10.10.Ph.D.2017.As.U
100 0 _aAsmaa Sami Mohammed Mansour
245 1 0 _aUnmodified gold nanoparticles for recognition of enterotoxin genes and other genes of Staphylococcus aureus isolated from bovine milk /
_cAsmaa Sami Mohammed Mansour ; Supervised Wagih A. Gad Elsaid , Sabry D. Morgan , Mahmoud D. Elhariri
246 1 5 _aجزيئات النانو للذهب الغير معدلة للتعرف على جينات السموم المعوية و جينات أخرى للميكروب العنقودى الذهبى المعزول من ألبان الأبقار
260 _aCairo :
_bAsmaa Sami Mohammed Mansour ,
_c2017
300 _a139 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aThis study applied on 141 bovine raw milk samples. Each sample was subjected for bacteriological examination. This work explored the high prevalence of S. aureus in raw milk samples. Out of 141 samples of raw milk studied; 24 of them were contaminated by S. aureus, corresponding to 17% of samples contaminated. Molecular identification of S. aureus isolates was completed using PCR revealing that 23 out of 24 isolates contained nuc gene with a percentage 95.8%. The risk of poisoning from ingestion of milk containing enterotoxins from S. aureus is well known so the enterotoxin production by S. aureus isolates was examined. It was evident that of 23 S. aureus isolates; 6 (26.1%) proved to be enterotoxigenic while non-enterotoxigenic isolates constituted 17 (73.9%). Testing of the isolates for the presence of nuc and coa genes, using unmodified gold nanoparticles, reported that all 23 isolates of S. aureus expressed nuc and coa genes
530 _aIssued also as CD
653 4 _aEnterotoxin genes
653 4 _aS. aureus
653 4 _aUnmodified gold nanoparticles
700 0 _aMahmoud Dardery Elhariri ,
_eSupervisor
700 0 _aSabry Darwish Morgan ,
_eSupervisor
700 0 _aWagih A. Gad Elsaid ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c65454
_d65454