000 | 02478cam a2200349 a 4500 | ||
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003 | EG-GiCUC | ||
005 | 20250223031944.0 | ||
008 | 180317s2017 ua dh f m 000 0 eng d | ||
040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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041 | 0 | _aeng | |
049 | _aDeposite | ||
097 | _aPh.D | ||
099 | _aCai01.10.10.Ph.D.2017.As.U | ||
100 | 0 | _aAsmaa Sami Mohammed Mansour | |
245 | 1 | 0 |
_aUnmodified gold nanoparticles for recognition of enterotoxin genes and other genes of Staphylococcus aureus isolated from bovine milk / _cAsmaa Sami Mohammed Mansour ; Supervised Wagih A. Gad Elsaid , Sabry D. Morgan , Mahmoud D. Elhariri |
246 | 1 | 5 | _aجزيئات النانو للذهب الغير معدلة للتعرف على جينات السموم المعوية و جينات أخرى للميكروب العنقودى الذهبى المعزول من ألبان الأبقار |
260 |
_aCairo : _bAsmaa Sami Mohammed Mansour , _c2017 |
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300 |
_a139 P. : _bcharts , facsimiles ; _c25cm |
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502 | _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology | ||
520 | _aThis study applied on 141 bovine raw milk samples. Each sample was subjected for bacteriological examination. This work explored the high prevalence of S. aureus in raw milk samples. Out of 141 samples of raw milk studied; 24 of them were contaminated by S. aureus, corresponding to 17% of samples contaminated. Molecular identification of S. aureus isolates was completed using PCR revealing that 23 out of 24 isolates contained nuc gene with a percentage 95.8%. The risk of poisoning from ingestion of milk containing enterotoxins from S. aureus is well known so the enterotoxin production by S. aureus isolates was examined. It was evident that of 23 S. aureus isolates; 6 (26.1%) proved to be enterotoxigenic while non-enterotoxigenic isolates constituted 17 (73.9%). Testing of the isolates for the presence of nuc and coa genes, using unmodified gold nanoparticles, reported that all 23 isolates of S. aureus expressed nuc and coa genes | ||
530 | _aIssued also as CD | ||
653 | 4 | _aEnterotoxin genes | |
653 | 4 | _aS. aureus | |
653 | 4 | _aUnmodified gold nanoparticles | |
700 | 0 |
_aMahmoud Dardery Elhariri , _eSupervisor |
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700 | 0 |
_aSabry Darwish Morgan , _eSupervisor |
|
700 | 0 |
_aWagih A. Gad Elsaid , _eSupervisor |
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856 | _uhttp://172.23.153.220/th.pdf | ||
905 |
_aNazla _eRevisor |
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905 |
_aSamia _eCataloger |
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942 |
_2ddc _cTH |
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999 |
_c65454 _d65454 |