000			02761cam a2200325 a 4500
003			EG-GiCUC
008			180329s2017 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.17.M.Sc.2017.Sh.D
100	0		_aShimaa Mousleh Sayed Ghoniem
245	1	0	_aDevelopment of multiplex real time PCR assay for detection of some equine respiratory viruses / _cShimaa Mousleh Sayed Ghoniem ; Supervised Hussein Aly Hussein Ahmed , Ayman Hany Eldeeb , Mohamed Galal Aggour
246	1	5	_aتطوير أختبار البلمره المتسلسل الحقيقي الوقتي المتعدد للكشف عن بعض الفيروسات التنفسية للفصيلة الخيلية
260			_aCairo : _bShimaa Mousleh Sayed Ghoniem , _c2017
300			_a156 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Virology
520			_aViral respiratory diseases are frequently reported in equine species affecting equine industry and cause huge economic losses. In the present study, we developed a multiplex assay for the simultaneous detection of the main viruses that cause respiratory diseases in equine species, Equine herpesvirus type 1(EHV-1) and 4 (EHV-4) and Equine influenza virus (EIV). The primers and probes amplified only the targeted viruses and there were no inter- assay cross amplifications or non-specific interactions. The multiplex assay efficiencies were 92.5%, 97% and 90% and the monoplex efficiencies were 97.4%, 98.2% and 90.7% for EHV-1, EHV-4 and EIV, respectively. The R square values (Rsq) in both forms were greater than 0.990. The performance of the assay was evaluated by analysing 152 different clinical samples from clinically infected horses. EHV-1 DNA was detected as the single causative agent in 12 samples, EHV-4 DNA in 9 samples and both EHV-1 and EHV-4 were detected in 4 samples. EIV RNA was not detected during this study. The accuracy of the assay was confirmed by comparing these results with those obtained from analysing the same samples using commercial rPCR and rRT- PCR diagnostic kits. This multiplex assay is proven to be a sensitive, specific, accurate and cost- effective method for the detection of the target viruses whether they occur as a single agent or as a part of co-infections
530			_aIssued also as CD
653		4	_aEquine
653		4	_aMultiplex real
653		4	_aTime PCR
700	0		_aAyman Hany Eldeeb , _eSupervisor
700	0		_aHussein Aly Hussein Ahmed , _eSupervisor
700	0		_aMohamed Galal Aggour , _eSupervisor
905			_aEnas _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c65628 _d65628