000 03010cam a2200337 a 4500
003 EG-GiCUC
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008 180404s2017 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.11.19.M.Sc.2017.Ma.D
100 0 _aMarwa Mohammed Mohammed Ghobashy
245 1 0 _aDetection of carbapenemase - producing enterobacteriaceae by the rapidec CARBA NP test /
_cMarwa Mohammed Mohammed Ghobashy ; Supervised Amal Shafik Balbaa , Basma Ahmed Elawady
246 1 5 _aالكشف عن البكتريا المعويه المنتجه لانزيم الكاربابيناميز عن طريق استخدام اختبار الرابيدك كاربا ان بي
260 _aCairo :
_bMarwa Mohammed Mohammed Ghobashy ,
_c2017
300 _a104 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
520 _aIntroduction: Rapid dissemination of carbapenem resistant Enterobacteriaceae represents a major threat to patient care and public health. Rapid detection and identification provides effective treatment and proper infection control measures. Aim of the work: The purpose of the study was to detect carbapenemase-producing Enterobacteriaceae with the Rapidec carba np test. Materials and methods: The study was conducted on 101 clinical samples. Sixty Enterobacteriaceae isolates were identified to species level by the API 20E then screened for carbapenemase-production by ertapenem disk diffusion method and Minimum inhibitory concentration methods. Confirmation was done by the Modified Hodge Test and the Rapidec carba np test. Results: Among the 60 Enterobacteriaceae isolates, E.coli was the most commonly encountered (51.6%), followed by K. pneumoniae (38.3%), K. oxytoca (3.3%), E. cloacae (1.6%) and P. mirabilis (5%). Out of the 60 isolates, 25 isolates (41.6%) were screened to be carbapenem resistant by ertapenem disk diffusion (ETP) and 35 isolates (58.3%) were sensitive. Twenty seven isolates (45%) were screened positive by MIC and 33 isolates (55%) were negative. Twenty five isolates (41.6%) were confirmed to be carbapenem resistant by the Modified Hodge test (MHT) and 35 isolates (58.3%) were sensitive. Twenty seven isolates (45%) were confirmed to be carbapenem resistant by the Rapidec carba np test and 33 isolates (55%) were sensitive. Conclusions: The Rapidec carba np test overall sensitivity was 100% and specificity was 94.29% in detecting carbapenemase producing Enterobacteriaceae isolates
530 _aIssued also as CD
653 4 _aEnterobacteriaceae
653 4 _aModified Hodge Test
653 4 _aRapidec CARBA NP test
700 0 _aAmal Shafik Balbaa ,
_eSupervisor
700 0 _aBasma Ahmed Elawady ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c65756
_d65756