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008 180417s2017 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.11.07.M.Sc.2017.Ya.I
100 0 _aYasmine Rabie Hashem Youssef Elammary
245 1 0 _aInterleukin-1Ý and interleukin-6 gene polymorphisms in sickle cell disease patients /
_cYasmine Rabie Hashem Youssef Elammary ; Supervised Shahira Zayed , Mona Elghamrawy , Nadia Ibrahim Sewelam
246 1 5 _aفى مرضى أنيميا الخلايا المنجليه Interleukin-1Ý and interleukin-6 التغيّرات الجينيه فى
260 _aCairo :
_bYasmine Rabie Hashem Youssef Elammary ,
_c2017
300 _a184 P. :
_bcharts ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
520 _aBackground: Sickle cell disease (SCD) is a disorder characterized by a heterogeneous clinical outcome. In the present study, we investigated the associations between Interleukin-1Ý (IL-1Ý) +3954 C>T and Interleukin-6 (IL-6) (-174G>C and -597 G>A) gene polymorphisms and clinical and laboratory data in SCD patients. Methods: The study was conducted on 100 SCD patients (59 sickle cell anemia patients 2SS3 and 41 sickle beta thalassemia patients 2SÝ3). Fifty age and sex matched healthy volunteers were included in the current study as a control group. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique was performed for the detection of IL-1Ý and IL-6 gene mutations. Results: In the present work, the homomutant genotypes of IL-1Ý (+3954 C>T), IL-6 (-174G>C) and IL-6 (-597 G>A) were higher in SS patients when compared to control group but with no significant differences. These genotypes were also higher in SS patients when compared to SÝ patients with no significant differences. As regards the clinical complications, the mutant genotypes of IL-1Ý (+3954 C>T) had a higher frequency of splenomegaly in SÝ patients. The mutant genotypes of IL-6 (-174G>C) and IL-6 (-597 G>A) had a higher frequency of leg ulcers in SS patients. Conclusion: From these results we can conclude that IL-1Ý (+3954 C>T), IL-6 (-174G>C) and IL-6 (-597G>A) polymorphisms are not associated with susceptibility to the disease; however, they are associated with some SCD complications
530 _aIssued also as CD
653 4 _aInterleukin-1Ý
653 4 _aInterleukin-6 gene
653 4 _aSickle cell disease patients
700 0 _aMona Elghamrawy ,
_eSupervisor
700 0 _aNadia Ibrahim Sewelam ,
_eSupervisor
700 0 _aShahira Zayed ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c65929
_d65929