| 000 | 03404cam a2200349 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223032006.0 | ||
| 008 | 180516s2017 ua dh f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.11.07.Ph.D.2017.He.R | ||
| 100 | 0 | _aHend Hamed abdellatif Tamim | |
| 245 | 1 | 0 |
_aRole of circulating miR-182 and miR-150 as noninvasive biomarkers for hepatocellular carcinoma / _cHend Hamed abdellatif Tamim ; Supervised Noha Mohamed Hosni Shaheen , Naglaa Ali Zayed , Rania Hassan khalifa |
| 246 | 1 | 5 | _a دورهما كدلالات لسرطان الكبد miR-15omiR-182 أهمية |
| 260 |
_aCairo : _bHend Hamed abdellatif Tamim , _c2017 |
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| 300 |
_a182 P. : _bcharts , facimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology | ||
| 520 | _aBackground: Hepatocellular carcinoma is one of the most common prevalent cancers worldwide. The incidence is highest where hepatitis C and hepatitis B are endemic. Hepatitis C virus is an independent risk factor for chronic hepatitis, liver cirrhosis, HCC and a major reason for liver transplantation. Current diagnostic methods show poor performance. Although serum Alpha fetoprotein has mainly been used for diagnosis and monitoring of HCC, its sensitivity and specificity are still not satisfying. Sensitive and specific cancer biomarkers are essential for early detection and diagnosis of HCC. MicroRNAs have been reported to be promising biomarkers for diagnosing cancers. This study was conducted to detect the role of miR-182 and miR-150 in diagnosis of HCC. Methods: The expression of miR-182 and miR-150 was evaluated using real-time quantitative RT-PCR in 120 patients: 40 HCC patients, 40 hepatitis C patients (20 cirrhotic and 20 non-cirrhotic) and 40 healthy controls. Results: Statistically significant down regulation of miR-182 and miR-150 in HCC when compared to HCV non-cirrhotic group (p=0.015, p=0.006 repectively). Statistically significant reduction of miR-150 in HCC when compared to the control (p=0.039). Statistically significant down regulation of miR-182 and miR-150 in HCV cirrhotic when compared to HCV non-cirrhotic group (p=0.003, p=0.024 respectively). Finally statistically significant upregulation of miR-182 in HCV non-cirrhotic when compared to the control (p=0.036). AFP at the cut-off value of 400 ng/ml showed sensitivity 15% for HCC. The capability for differential diagnosis of HCC and HCV non-cirrhotic was analyzed by combining miR-182, miR-150 and AFP and this resulted in improved sensitivity (90%) and increased diagnostic accuracy (80%) Conclusion: miR-182 and miR-150 can be used as non invasive biomarkers for hepatocellular carcinoma as well as predictive markers for detection of cirrhosis progression in HCV infected patients. miR-182 may also be useful as circulatory marker for HCV infection | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aHepatitis C virus | |
| 653 | 4 | _aHepatocellular carcinoma | |
| 653 | 4 | _amiR-182 | |
| 700 | 0 |
_aNaglaa Ali Zayed , _eSupervisor |
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| 700 | 0 |
_aNoha Mohamed Hosni Shaheen , _eSupervisor |
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| 700 | 0 |
_aRania Hassan khalifa , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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| 905 |
_aShimaa _eCataloger |
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| 942 |
_2ddc _cTH |
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_c66218 _d66218 |
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