000			03393cam a2200325 a 4500
003			EG-GiCUC
008			180530s2017 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.09.02.Ph.D.2017.Mo.D
100	0		_aMostafa Ibrahim Negm
245	1	0	_aDetermination of mesenchymal stem cells origin during regenerative endodontic procedure with assessment of the ability of an induced blood clot to aid in maturogenesis of immature necrotic permanent teeth : _bA clinical & radiographic study / _cMostafa Ibrahim Negm ; Supervised Jealan Mohamed Elshafei , Nagwa Roshdy , Ghada Elhilaly Eid
246	1	5	_aتحديد منشأ خلايا الميزينكيم الجذعية خلال علاج الجذور التصليحي مع تقدير قابلية الجلطة الدموية المستنتجة في المساعدة علي نضوج جذر السنة الدائمة الغير ناضجة : _bدراسة اكلينيكية و اشاعية
260			_aCairo : _bMostafa Ibrahim Negm , _c2017
300			_a123 P. : _bcharts , facimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University -Faculty of Oral and Dental Medicine - Department of Endodontics
520			_aThe current study was conducted to determine the origin of stem cells during treatment of necrotic immature permanent teeth using blood clot regenerative endodontic procedure, and whether apical radiolucency has any negative influence on the treatment outcome both clinically and radiographically. Thirty teeth of twenty-four patients with necrotic immature permanent teeth were included in the current study which, were divided into two groups according to the presence or absence of periapical radiolucency. All patients were subjected to regenerative endodontic standardized protocol including minimal instrumentation, chemical disinfection using 1.5% NaOCl irrigant and Bimix intracanal medicament for 3 weeks. After subsidence of signs and symptoms, Bimix was removed by NaOCl irrigant followed by chelation using 17% EDTA for growth factors exposure and finally saline was dispensed inside root canals. Quantitative assessment of mesenchymal stem cells origin was conducted using molecular biology techniques, through investigating the gene expression of CD73 and CD105 using real-time RT- PCR. Saline samples were taken on sterile paper points just before bleeding induction and blood samples were taken immediately after irritation of periapical tissues using endodontic file to decide whether stem cells arise from disinfected pulp canal space or from periapical tissues delivered via bleeding induction. Comparison between the two groups showed the existence of both gene markers in both groups with and without radiolucencies as well as both saline and blood samples, however, with variable RQ values. Paired t-test was used for comparing the RQ values for both CD105 and CD73 in both saline and blood samples. In both
530			_aIssued also as CD
653		4	_aBlood Clot
653		4	_aImmature Necrotic Permanent Teeth
653		4	_aRegenerative Endodontic Procedure
700	0		_aGhada Elhilaly Eid, _eSupervisor
700	0		_aJealan Mohamed Elshafei , _eSupervisor
700	0		_aNagwa Roshdy , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c66400 _d66400