000			02690cam a2200313 a 4500
003			EG-GiCUC
008			180624s2017 ua a f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.10.M.Sc.2017.Wa.A
100	0		_aWagdy Samir Beshir Youssef
245	1	0	_aAssessment of some mycobacterial antigens in diagnosis of bovine tuberculosis / _cWagdy Samir Beshir Youssef ; Supervised Kamelia Mahmoud Osman , Emad Mokhtar Riad
246	1	5	_aتقييم بعض انتيجينات الميكوبكتريا فى تشخيص مرض السل البقرى
260			_aCairo : _bWagdy Samir Beshir Youssef , _c2017
300			_a208 P. : _bill ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aIn this study, some M. bovis cell extract antigens 2sonicated antigen (SA)3, culture filtrate antigens "short term culture filtrate (ST-CF)" and tuberculin were prepared from 29 mycobacterium isolates after the bacteriological examination of 39 tuberculin positive animals which tested from Elsharkia and Elmenofia governorates by cultural methods. Microscopic examination was conducted for all 39 tuberculous lesions by using Ziehl-Nelseen stain and 27 were positive with a percentage of 69.23%. Real time PCR was carried out on all 29 isolates for identification of recovered colonies and all were identified as Mycobacterium bovis with a percentage of 100%. Extraction of M. bovis with sonication resulted in the recovery of 1.3 mg/ml of soluble protein in the sonicated extract and the protein concentration of M. bovis ST-CF at 2nd week of growth was 2.7 mg/ ml. In addition, the protein concentration of M. bovis prepared tuberculin was 2.0 mg/ml and 1.0 mg/ml of offered PPD. SDS-PAGE was applied for characterization of antigenic extracts and CF of M. bovis and Western blotting analysis was performed. The use of ELISA on serum samples collected from the 1540 cows, before being tested by SICTT, revealed that 131 (8.51%) animals were positive to bTB by using PPD, 139 (9.03%) by using the commercial mixture antigen, 120 (7.79%) by using SA, 125 (8.12%) by using ST-CF and 133 (8.64%) positive animals by using prepared tuberculin, indicating that the use of mixture antigen could help to give more accurate diagnosis
530			_aIssued also as CD
653		4	_aAntigens
653		4	_aBovine tuberculosis
653		4	_aELISA
700	0		_aEmad Mokhtar Riad , _eSupervisor
700	0		_aKamelia Mahmoud Osman , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c66616 _d66616