| 000 | 02896cam a2200349 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223032018.0 | ||
| 008 | 180626s2017 ua do f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.08.06.Ph.D.2017.Ma.M | ||
| 100 | 0 | _aMarwa Omar Hussein Elnahas | |
| 245 | 1 | 0 |
_aMicrobiological and physiological studies on the production of polysaccharides from local actinomycetes / _cMarwa Omar Hussein Elnahas ; Supervised Magdy Ali Amin , Judy D. Wall , Mohamed Magd Eldin Hussein |
| 246 | 1 | 5 | _aدراسات ميكروبيولوجية و فيسيولوجية على إنتاج السكريدات العديدة من الأكتنوميستات المحلية |
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_aCairo : _bMarwa Omar Hussein Elnahas , _c2017 |
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_a197 P. : _bcharts , photographs ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology | ||
| 520 | _aTwo streptomyces strains were isolated from soil samples and the sequence of ~ 1500 nucleotides of their 16S rDNA showed 99% identity to streptomyces sp. HV10 and 100% identity to streptomyces chattanoogensis respectively. These newly isolated Streptomyces strains produced extracellular polysaccharides (EPS) named MOE6-EPS and MOE7-EPS respectively. The two EPS were characterized by various spectroscopic techniques including fourier transform infrared (FTIR), HPLC, 1HNMR as well as UV visible spectroscopy. MOE6-EPS composed of 71±1% carbohydrate by weight, 3.6±0.1% uronic acid and 0.78±0.1% protein, while MOE7-EPS composed of 98±0.5% carbohydrate by weight, 0.2±0.1% uronic acid and 0.3±0.1% protein. MOE6-EPS and MOE7-EPS showed antioxidant activities by scavenging the hydroxyl free radical scavenging activity and 2,2-diphenyl-2-picryl-hydrazyl (DPPH) radicals. In addition, MOE6-EPS showed high ferrous ion (Fe2+) chelation activity which is another antioxidant activity. Interestingly, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays that were colorimetric assays for NAD(P)H-dependent cellular oxidoreductases and a proxy of the number of viable cells, showed that MOE6-EPS and MOE7-EPS inhibited the proliferation of the human breast cancer cells (MDA-MB-231). Moreover, the scratch wound assay showed that MOE6-EPS and MOE7-EPS reduced the migration of human breast cancer cells (MDA-MB-231) in EPS concentration dependent manner | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aAntioxidant activities | |
| 653 | 4 | _aPolysaccharides | |
| 653 | 4 | _aStreptomyces | |
| 700 | 0 |
_aJudy D. Wall , _eSupervisor |
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| 700 | 0 |
_aMagdy Ali Amin , _eSupervisor |
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| 700 | 0 |
_aMohamed Magd Eldin Hussein , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aSamia _eCataloger |
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_2ddc _cTH |
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_c66629 _d66629 |
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