000			03119cam a2200349 a 4500
003			EG-GiCUC
005			20250223032036.0
008			180830s2017 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.28.Ph.D.2017.Ka.M
100	0		_aKariman Hassan Elhamamsy
245	1	0	_aMicroRNAs as a potential diagnostic new biomarkers in diagnosis of sepsis in pediatric patients / _cKariman Hassan Elhamamsy ; Supervised Iman Ahmed Ehsan Abdelmeguid , Lobna Mohamed Anas Fawaz , Olfat Gamil Shaker
246	1	5	_aكعلامات بيولوجية تشخيصية جديدة فى تشخيص تسمم الدم فى مرضى الأطفال microRNAs استخدام ال
260			_aCairo : _bKariman Hassan Elhamamsy , _c2017
300			_a180 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Pediatrics
520			_aBackground: Sepsis represents the main cause of mortality at intensive care units. The mortality rate for all septic episodes is 30{u2013}50%. miRNAs profile data could be beneficial as a specific diagnostic biomarker for the diagnosis of sepsis and systemic inflammatory response syndrome. The aim of this study was to access the role of certain miRNAs (miRNA-122, miRNA-181b, miRNA-223 and miRNA-146a) in diagnosis of sepsis and assess the probability of these miRNAs in prediction of poor prognosis and mortality. This could provide a potentially novel biomarker for sepsis detection and therapeutic strategies. Patients and methods: This was a prospective case control study of patients admitted to Cairo university pediatric intensive care unit (PICU). Forty cases (age: 1-24 months), 60% males and 40% females were compared to 40 healthy controls that were matched for age and sex. In addition to routine laboratory tests, cultures from different sites and imaging were done according to their need, special blood samples were collected for determination of miRNA by quantitative real time PCR technique. Results: miRNA-122 (19.5 ±29.8) and miRNA-181b (3.5 ±1.69) were found to be very sensitive and specific in diagnosing sepsis by their up-regulation early during septic conditions (p<0.001). While miRNA-223 (0.55 ±0.28) and miRNA-146a (0.15 ±0.10) are also very specific and highly sensitive in diagnosing sepsis conditions but by their down-regulation (p<0.001). We also found that only serum level of miRNA-223 can be used for poor prognosis prediction in sepsis with sensitivity=70% and specificity=75%, while the other three miRNAs could not predict poor prognosis during sepsis
530			_aIssued also as CD
653		4	_aMicroRNA (miRNA)
653		4	_aPediatric intensive care unite
653		4	_aSepsis
700	0		_aIman Ahmed Ehsan Abdelmeguid , _eSupervisor
700	0		_aLobna Mohamed Anas Fawaz , _eSupervisor
700	0		_aOlfat Gamil Shaker , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c67204 _d67204