000			02778cam a2200337 a 4500
003			EG-GiCUC
005			20250223032038.0
008			180903s2018 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.08.09.M.Sc.2018.Ri.E
100	0		_aRiham Mohamed Karkeet
245	1	0	_aEffect of an epigenetic modifier as an anticancer drug on colorectal cancer cell lines / _cRiham Mohamed Karkeet ; Supervised Hanan S. Elabhar , Samia A. Shouman
246	1	5	_aتأثير مغير التخلق المتوالي كمضاد للسرطان في خطوط خلايا سرطان القولون والمستقيم
260			_aCairo : _bRiham Mohamed Karkeet , _c2018
300			_a122 P. : _bcharts ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology
520			_aCancer is a multifactorial disease and its treatment requires combining different drugs to act synergistically. Epigenetic factors play a key role in cancer etiology and drug response. Modification of these epigenetic changes is a promising avenue for the development of new and effective therapies. 5-Fluorouracil (5-FU), an anti-metabolite, is the gold standard in treatment of colorectal cancer and valproic acid (VPA) is a histone deacetylase inhibitor, thus it has an epigenetic modifying effect. The aim of the current study was to investigate the epigenetic modifying effect of valproic acid (VPA) on 5-florouracil (5-FU) cytotoxic effect in CRC cell lines (Caco2 and HCT 116) and explore the possible underlying mechanism(s) of their interaction. Different combination regimens of 5-FU and VPA were analyzed using the factorial design. The maximum inhibitory combinations, as well as the minimum inhibitory combinations, were chosen to study the differential effect of VPA on various epigenetic markers as global DNA methylation ratio, histone acetyl transferase (HAT) activity and histone 3 (H3) acetylation percentage. Moreover, different metastatic parameters were studied as vascular endothelial growth factor level, matrix metalloproteinases (MMPs) activity and SNAI1 protein expression. Apoptosis was assessed by measuring Caspase-3 level, Caspase-9, ITPK1, AKT and Bcl-2 protein expression. Also, thymidine synthetase (TS) level was determined in cell culture medium
530			_aIssued also as CD
653		4	_aAnticancer drug
653		4	_aColorectal cancer cell lines
653		4	_aEpigenetic modifier
700	0		_aHanan S. Elabhar, _eSupervisor
700	0		_aSamia A. Shouman , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c67283 _d67283