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003 EG-GiCUC
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008 180903s2017 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.09.14.Ph.D.2017.La.I
100 0 _aLayla Hafed Abdulla Fadhel
245 1 0 _aIncidence of candida albicans in oral squamous cell carcinoma with and without lymph nodes metastasis in comparison with oral dysplasia using PAS stain and PCR :
_bIn vitro study /
_cLayla Hafed Abdulla Fadhel ; Supervised Heba Ahmed Farag , Dalia Hussein Elrouby , Olfat Gamil Shaker
246 1 5 _aالإصابة بالمبيضات البيض في سرطان الخلايا الحرشفية الفموي مع وبدون الإنتشار للعقد الليمفاوية في مقارنة مع النمو الفموي الشاذ باستخدام صبغة الحمض الدوري شيف وتفاعل البوليميريز المتسلسل :
_bدراسة مخبرية
260 _aCairo :
_bLayla Hafed Abdulla Fadhel ,
_c2017
300 _a108 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology
520 _aCandida hyphae and spores were detected by PAS in 11 specimens (20.3 %) of total 54 studied specimens. This was confirmed by the morphological appearance of rods having magenta color. Using RT-PCR, CaADH1 mRNA was detected in 29 specimens of total 54 studied specimens (53.7 %). This was confirmed by the appearance of band at the same level of the marker identical in length to the CaADH1 mRNA gene. All control group specimens were negative for Candida hyphae, spores and CaADH1 mRNA. Candida hyphae and spores were detected by PAS in only one of the oral dysplasia specimens and the same case was positive for CaADH1 mRNA by RT-PCR. In OSCC without lymph node metastasis group, 5 out of the 16 studied specimens in that group were positive by PAS, whereas the whole 16 specimens were positive by RT-PCR. Moreover, in OSCC with lymph node metastasis, 5 out of 15 studied specimens in that group were positive by PAS, whereas the whole 12 specimens were positive by RT-PCR
530 _aIssued also as CD
653 4 _aPAS stain
653 4 _aPCR
653 4 _aRT-PCR
700 0 _aDalia Hussein Elrouby ,
_eSupervisor
700 0 _aHeba Ahmed Farag ,
_eSupervisor
700 0 _aOlfat Gamil Shaker ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c67287
_d67287