| 000 | 02616cam a2200325 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 008 | 180918s2018 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
||
| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.08.06.Ph.D.2018.Ne.S | ||
| 100 | 0 | _aNesreen Ahmed Safwat | |
| 245 | 1 | 2 |
_aA Study on the potential in vitro antimycobacterial activity of natural product(s) from selected wild plants / _cNesreen Ahmed Safwat ; Supervised Mohammed A. Ramadan , Ramy K. Aziz , Khadegah F. Amer |
| 246 | 1 | 5 | _aدراسة علي الفاعلية المعملية المحتملة المضادة للميكوباكتيريام لمنتجات طبيعية المستخرجة من نباتات برية مختارة |
| 260 |
_aCairo : _bNesreen Ahmed Safwat , _c2018 |
||
| 300 |
_a156 P. : _bcharts , facsimiles ; _c25cm |
||
| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology | ||
| 520 | _aTuberculosis remains a major health problem accentuated by the rise of resistance to all available drugs. Therefore, this study was launched to discover novel antituberculosis agents from natural products.Twelve wild plants were in vitro screened for their activity against typical and atypical Mycobacterium species. The most active plant, Euphorbia paralias, was further fractionated with different organic solvents, and the activity of the obtained fractions was determined by agar diffusion and broth microdilution methods.The methanol fraction was found to be the most active against Mycobacterium spp.,and was non-toxic in doses up to 10 g/kg of animal weight. The main component was separated by column chromatography then identified by ultraviolet spectroscopy and nuclear magnetic resonance analysis as quercetin-3-O-Ý-D-glucoside. Treatment with quercetin-3-O-Ý-D-glucoside inhibited cell survival of HepG2 and A549 cells in a dose and time dependent manner. Molecular docking analysis suggested that quercetin-3-O-Ý-D-glucoside inhibits the glutamine synthetase enzyme, a promising target for the development of drugs for tuberculosis therapy. This current study prediction was confirmed by the in vitro glutamine synthetase biosynthesis assay | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aEuphorbia paralias | |
| 653 | 4 | _aMycobacterium | |
| 653 | 4 | _aTuberculosis | |
| 700 | 0 |
_aKhadegah F. Amer , _eSupervisor |
|
| 700 | 0 |
_aMohammed A. Ramadan , _eSupervisor |
|
| 700 | 0 |
_aRamy K. Aziz , _eSupervisor |
|
| 905 |
_aNazla _eRevisor |
||
| 905 |
_aShimaa _eCataloger |
||
| 942 |
_2ddc _cTH |
||
| 999 |
_c67512 _d67512 |
||