000			03700cam a2200349 a 4500
003			EG-GiCUC
005			20250223032045.0
008			180918s2018 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.16.Ph.D.2018.As.C
100	0		_aAsmaa Mohamed Abdelhameed
245	1	2	_aA comparative histological study on the effect of adipose-derived stem cells versus their conditioned medium on indomethacin induced enteritis in adult female albino rats / _cAsmaa Mohamed Abdelhameed ; Supervised Abeer Fouad Abdelmohsen , Nagla Mohamed Salama , Laila Ahmed Rashed
246	1	5	_aدراسة هستولوجية مقارنة علي تأثير الخلايا الجذعية المستخلصه من النسيج الدهني مقابل الوسط المكيف لها في التهاب الأمعاء الدقيقه المستحث تجريبيا بالإندوميثاسين في إناث الجرذان البيضاء البالغه
260			_aCairo : _bAsmaa Mohamed Abdelhameed , _c2018
300			_a200 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Histology
520			_aBackground and Aim of the work: Inflammatory bowel disease, including Crohn{u2019}s disease (CD), is a chronic complex immune-inflammatory disorder with increasing prevalence and inadequate conventional treatment. So, this work was conducted to evaluate the therapeutic effect of adipose-derived mesenchymal stem cells (ADSCs) versus ADSC-conditioned medium (CM) in a rat model of enteritis (Crohn{u2019}s disease). Materials and Methods: Fifty-six female albino rats were used in this study, 16 rats served as control (group I). In the remaining 40 rats, enteritis was induced by SC injection of 2 doses of indomethacin 7.5 mg/kg, on days 0 &1 of the experiment.They were left until day 10 then divided equally into 5 groups: Groups II & III received no further treatment and sacrificed at days 10 and 24 respectively. Groups IV,V &VI were treated with ADSCs (1{u00D7}106 cells, IV single dose ), ADSC-CM (0.5ml IP, 5 doses) and DMEM (0.5ml IP, 5 doses) respectively; they were all sacrificed at day 24. Ileum specimens were taken from all animals and stained with H&E, Masson's trichrome & Alcian blue/ PAS stains as well as MMP1 and PCNA immunostains. Morphometric measurements of area % of collagen & MMP1, number of goblet cells & PCNA positive cells as well as optical density of Alcian blue/ PAS reaction were taken and statistically analyzed.Results: Groups II, III and VI revealed obvious damage of villi and crypts, desquamation of epithelium, oedema and obvious mononuclear cellular infiltration together with significant decrease in number of goblet cells & PCNA +ve cells as well as in the optical density of Alcian blue/ PAS reaction and significant increase in the area % of collagen (only in groups III&VI) and MMP1 as compared to control. Groups IV and V nearly regained the normal histological structure of villi & crypts with significant increase in number of goblet cells & PCNA +ve cells as well as in the optical density of Alcian blue/ PAS reaction and significant decrease in the area % of collagen and MMP1
530			_aIssued also as CD
653		4	_aCrohn{u2019}s disease
653		4	_aEnteritis
653		4	_aIndomethacin
700	0		_aAbeer Fouad Abdelmohsen , _eSupervisor
700	0		_aLaila Ahmed Rashed , _eSupervisor
700	0		_aNagla Mohamed Salama , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c67518 _d67518