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| 003 | EG-GiCUC | ||
| 005 | 20250223032047.0 | ||
| 008 | 180922s2018 ua dh f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.11.03.M.Sc.2018.Ma.E | ||
| 100 | 0 | _aManar Monir Youssef Amin | |
| 245 | 1 | 4 |
_aThe effect of mesenchymal stem cells derived microvesicles on the treatment of experimental CCL₄ induced liver fibrosis in rats / _cManar Monir Youssef Amin ; Supervised Dina Sabry Abdelfatah , Abbas Mohamed Abbas , Heba Abdelmonem Ibrahim |
| 246 | 1 | 5 | _aتأثير الحويصلات الدقيقة المستخلصة من الخلايا الجذعية الميشنزمية على علاج التليف الكبدى الناجم عن حقن رباعى كلوريد الكربون فى فئران التجارب |
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_aCairo : _bManar Monir Youssef Amin , _c2018 |
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_a118 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Medical Biochemistry | ||
| 520 | _aBackground &Aim:Stem cell-based therapies especially the mesenchymal stem cells (MSCs) have potential for treatment of liver injury by contributing to regenerative responses, through functional tissue replacement or paracrine effects. The release of microvesicles (MVs) from these cells has been implicated in intercellular communication, and may contribute to beneficial paracrine effects of stem cell-based therapies. The aim of our study is to investigate the effect of administration of murine bone marrowMSC-MVs(mBM-MSC-MVs) on the treatment of carbon tetrachloride (CCL₄) induced liver fibrosis in rats.Thereby, to determine their potential utility as therapeutic agents for tissue repair and functional restoration in liver fibrosis. Methods : Our work included: Preparation, isolation then identification of BM-MSCs in culture by morphology and flow cytometry, preparation then identification of BM-MSC-MVs by transmission electron microscopy and polymerase chain reaction (PCR) detection of Ý-integrin gene expression, then preparation of rat model of liver fibrosis by CCl4-induced injury, injection of prepared BM-MSC-MVs in injured rats then the effects of BM-MSC-MVs were evaluated by biochemical analysis of liver functions, RNA gene expression quantitation for collagen-1Ü, transforming growth factor Ý(TGF-Ý), interleukin-1Ý(IL-1Ý), vascular endothelial growth factor (VEGF) by real time reverse transcription PCR (RT-PCR) techniquesand finally histopathologicalanalysis of the liver tissues of all studied groups | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aLiver fibrosis | |
| 653 | 4 | _aMesenchymal stem cells | |
| 653 | 4 | _aMicrovesicles | |
| 700 | 0 |
_aAbbas Mohamed Abbas , _eSupervisor |
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| 700 | 0 |
_aDina Sabry Abdelfatah , _eSupervisor |
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| 700 | 0 |
_aHeba Abdelmonem Ibrahim , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aShimaa _eCataloger |
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_2ddc _cTH |
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_c67588 _d67588 |
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