000			02993cam a2200337 a 4500
003			EG-GiCUC
005			20250223032136.0
008			181222s2018 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.19.Ph.D.2018.Ha.I
100	0		_aHanaa Fathy Aly Abdelatty
245	1	0	_aImportant virulence factors and related genes in uropathogenic E. coli and their relation to fluoroquinolone resistance / _cHanaa Fathy Aly Abdelatty ; Supervised Magda Ibrahim Mohamed Ayoub , Noha Mahmoud Abdelrahim Gohar
246	1	5	_aعوامل الضراوة المهمة وجيناتها فى العصيات القولونية المسببة لعدوى المجارى البولية وعلاقتها بمقاومة المضاد الحيوى فلوروكينولون
260			_aCairo : _bHanaa Fathy Aly Abdelatty , _c2018
300			_a118 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
520			_aEscherichia coli(E. coli) is the predominant pathogen causing urinary tract infection. E. coli expresses a wide spectrum of virulence factors that enable it to cause the disease. The present study was conducted on 50 E. coli isolates to assess some important phenotypic virulence factors of E. coli: haemolytic activity, cytotoxic activity and haemagglutination and their relations to the following virulence genes: hlyA, cnf-1 and papC, by using multiplex PCR. Fluoroquinolones susceptibility was done using disk diffusion method.The results showed that 30% of the isolates were Ý- haemolytic, 18% of the isolates showed haemagglutination with human RBCs, with higher prevalence for MRHA (12%) than MSHA (6%), and 92% of the isolates showed morphological changes, detachment and lysis in HEp-2 cells. Multiplex PCR showed that the most prevalent gene was cnf-1(30%), while 26% of isolates were positive for hlyA and 26% of isolates were positive for papC. Only one UPEC isolate showed the simultaneous presence of papC, cnf-1 and hlyA while 10 isolates were harbouring hlyA and cnf-1, 2 isolates were harbouring cnf-1 and papC genes and 2 isolates were harbouring hlyA and papC simultaneously. We found that hlyA gene was significantly detected in the Ý- haemolytic than non- haemolytic isolates. As regard fluoroquinolones susceptibility, 60% of the isolates were resistant to the used fluoroquinolones with no significant difference between resistant and susceptible isolates as regard virulence factors and genes
530			_aIssued also as CD
653		4	_aEscherichia coli
653		4	_aMultiplex PCR
653		4	_aVirulence
700	0		_aMagda Ibrahim Mohamed Ayoub , _eSupervisor
700	0		_aNoha Mahmoud Abdelrahim Gohar , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c69192 _d69192