000			03114cam a2200349 a 4500
003			EG-GiCUC
005			20250223032144.0
008			190105s2018 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.11.07.Ph.D.2018.As.R
100	0		_aAsmaa Esmail Ali Nasr Eldin
245	1	0	_aRelative telomeric length and polymorphism in telomere maintenance gene telomerase reverse transcriptase in hepatocellular carcinoma patients / _cAsmaa Esmail Ali Nasr Eldin ; Supervised Enas Hamdy Mahmoud , Ahmed Ameen Emara Hekel , Alaa Afif Abdelrauof
246	1	5	_aالنسبة الطولية لتلومير وتعدد اشكال في عكس التلموريز الناسخ في الجينات في مرضي سرطان الكبد
260			_aCairo : _bAsmaa Esmail Ali Nasr Eldin , _c2018
300			_a112 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
520			_aBackground: Telomere length and telomerase activity are crucial for cancer initiation and the survival of tumors. Telomere length is maintained by telomerase in 90% of human cancers,while 10% of cancers use an alternative mechanism of telomere lengthening termed (ALT).Another mechanism for Cancer cells is through activating or upregulating the normally silent human TERT gene (hTERT) that encodes telomerase Objectives:The aim of the present study wasto assess the role of telomeric length in the development of hepatocellular carcinoma(HCC) and to study the effect of 2SNPs in TERT gene rs13167280, rs2736098 on telomere length Subjects and Methods: the study was conducted on a total number of 100 patients,subdivided into two groups HCC and HCV groups.telomeric length was measured using quantitative real time PCR(sybergreen),SNP were measuring using Taqman probe on step{u2013}one real time PCRResults:We found thatthe patients with HCC had a significant short relative telomere length(RTL)with median level 0.15 compared to the median of HCV group which was 1.03 with the p-value <0.001.The performance of Relative telomere length was detected using ROC Curve. At the cut-off value of 0.7, RTL showed sensitivity 92 %, specificity 94%, diagnostic accuracy 93% and the area under the curve (AUC) were 0.917, and there was no statistical significant difference observed on comparing the two SNPs in HCC and HCV groups Conclusions: the median value of RTL in HCC patients was short compared to HCV patients, so the relative telomeric length could be used as a non invasive biomarker for HCC
530			_aIssued also as CD
653		4	_aHepatocellular Carcinoma, ,
653		4	_aRelative telomeric length
653		4	_aTelomere maintenance gene
700	0		_aAhmed Ameen Emara Hekel , _eSupervisor
700	0		_aAlaa Afif Abdelrauof , _eSupervisor
700	0		_aEnas Hamdy Mahmoud , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c69413 _d69413