000			02839cam a2200325 a 4500
003			EG-GiCUC
008			190128s2018 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.07.12.Ph.D.2018.He.E
100	0		_aHeba Badr Soliman Ibrahim
245	1	0	_aEffect of culture media and irradiation on micropropagation and anatomical structure of Brunfelsiapauciflora plant / _cHeba Badr Soliman Ibrahim ; Supervised Salwa Salem Sakr , Afaf Mahmoud Alsayed Habib , Mamdouh Ahmed Elshamy
246	1	5	_aBrunfelsia pauciflora تأثير بيئة الزراعة والأشعاع علي الأكثار الدقيق والتشريح لنبات
260			_aCairo : _bHeba Badr Soliman Ibrahim , _c2018
300			_a189 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Ornamental Horticulture
520			_aThe aim of this study was to investigate the most suitable protocol for micropropagation of Brunfelsia pauciflora plant, as affected with type and strength of culture media supplemented with different growth regulators and study the effect of gamma irradiation on shooting behavior, rooting, acclimatization, chemical analysis, Anatomical studies and genetic fidelity of Brunfelsia pauciflora shoot. Micropropagation of Brunfelsia pauciflora: application of a sodium hypochlorite NaOCl (0.75%) for 20 min then 3 g/l mercuric chloride (HgCl2) for 1min. gave the best disinfection and the highest survival percentage (86.67%) of during surface sterilization. The sampling data in July resulted in the highest value for the number of shoots and tallest shoots on establishment stage. Using full MS medium supplemented with 3.0 g/l AC resulted in the highest shoot length, number of shoots performed and the highest value of leaves number. On multiplication stage using MS medium supplemented with 2mg/l of BA produced the maximum number of proliferated shoots while MS medium supplemented with 4mg/l BA gave the highest callus formation. The highest mean value of shoot length and number of leaves was recorded from using MS medium supplemented with 0.5 mg/l TDZ and 2.0 mg/l 2iP. For shoot regeneration from leaf explants, MS medium supplemented with 0.5 mg/l TDZ and 2.0 mg/l 2iP resulted the highest mean value of callus formation, shoots regeneration,shoot length and number of leaves
530			_aIssued also as CD
653		4	_aGrowth regulators
653		4	_aIrradiation
653		4	_aMicropropagation
700	0		_aAfaf Mahmoud Alsayed Habib , _eSupervisor
700	0		_aMamdouh Ahmed Elshamy , _eSupervisor
700	0		_aSalwa Salem Sakr , _eSupervisor
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c69797 _d69797