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008 190211s2018 ua dho f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.10.03.Ph.D.2018.En.A
100 0 _aEnas Sayed Abdelaziz
245 1 0 _aAdvanced studies on coccidiosis in broiler chicken flocks with special reference to methods of diagnosis :
_bprevention and control /
_cEnas Sayed Abdelaziz ; Supervised Medhat Abdelhadi Mohammed , Youssef Ibrahim Youssef , Samir Abdelmoez Abdelwanees
246 1 5 _aدراسات متقدمه عن الكوكسيديوزيس فى قطعان دجاج التسمين و باشاره خاصه الى طرق التشخيص :
_bالوقاية و السيطرة
260 _aCairo :
_bEnas Sayed Abdelaziz ,
_c2018
300 _a235 P. :
_bcharts , facsimiles , photographs ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Animal Diseases
520 _aTwenty-eight broiler chicken farms from 16 to 70 days of age were examined clinically for suspected outbreaks of coccidiosis in eight governorates in Egypt. Flock history, clinical signs, dropping score and lesion score were recorded at time of examination. Fecal as well as intestinal samples (from different intestinal portions) were examined for coccidiosis using direct wet smear. Positive samples subjected to DNA extraction followed by amplification of internal transcribed spacer 1(ITS1) region of Eimeria genome with species specific primers of E. tenella, E. necatrix, E. maxima and E. acervulina for detection of these species using conventional PCR.The ITS1 region of ribosomal DNA (rDNA) from highly positive sample of single infection with E. tenella was sequenced and compared with sequence of ITS1 region of E. tenella strain in coccivac-B Vaccine. Results revealed that, the highest mortality rate (2.49%) was recorded in cobb breed in Dakahlia governorate while the lowest mortality (0.5%) was recorded in avian species in Dakahlia and Damitta governorate.Mean lesion score revealed that higher score in different types of chickens attributed to cecal portion 0.6-3.3 and lower score was observed in other portions 0-0.4 in duodenal portion, 0-0.9 in jejunal portion and 0-0.6 in illiual portion.PCR results revealed the prevalence of E. tenella 100% then E. necatrix 28.57 % from positive cecal and fecal samples, E. acervulina 21.42% from positive intestinal and fecal samples
530 _aIssued also as CD
653 4 _aBroilers
653 4 _aCoccivac-B
653 4 _aProbiotic
700 0 _aMedhat Abdelhadi Mohammed ,
_eSupervisor
700 0 _aSamir Abdelmoez Abdelwanees
_eSupervisor
700 0 _aYoussef Ibrahim Youssef ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c70093
_d70093