000			02795cam a2200325 a 4500
003			EG-GiCUC
008			190224s2018 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.12.21.Ph.D.2018.Ya.M
100	0		_aYasmeen Mohamed Abodief Allam
245	1	0	_aMolecular genetic response to heat stress in cows-Bos taurus and river buffalo-Bubalus bubalis bubalis in Egypt / _cYasmeen Mohamed Abodief Allam ; Supervised Mohamed Akmal Elghor , Adel Khalil Ebrahim , Abdelmohsen Hammam
246	1	5	_aالاستجابة الوراثية الجزيئية للاجهاد الحرارى فى الأبقار- بوس تاوروس و الجاموس النهرى - بيوبالس بيوباليس بيوباليس فى مصر
260			_aCairo : _bYasmeen Mohamed Abodief Allam , _c2018
300			_a115 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Zoology
520			_aThe aim of the present work was to study the effect of heat stress on the ovarian intra-follicular microenvironment of the Egyptian cattle "Bos taurus" and buffalo "Bubalus bubalis bubalis" as a key stone into the reproduction process. Follicular fluid samples were collected by aspiration from medium sized ovarian follicles of Egyptian cattle "Bos taurus" and buffalo "Bubalus bubalis bubalis" during spring and summer seasons. Part of the follicular fluid samples were searched for oocytes and another part was stored at -80{u02DA}C for hormonal and gene expression analysis. The picked up oocytes were examined under stereomicroscope and divided according to their morphological state into four classes: A (excellent), B (good), C (fair) and D (poor). Total oocytes recovery rates were recorded as well as the percentages of each class. Oocytes of classes A and B were used for IVM and IVF, the oocytes maturation, fertilization, cleavage, morulae and blastocyst formation rates were recorded and data were statistically analyzed using ANOVA. The hormonal levels of progesterone and 17Ý-estradiol were estimated by the ELISA technique and statistically analyzed using student's t-test. The changes in mRNA expression levels of the FST, INHBA and ESR2 genes were determined using real-time PCR, the data were analyzed using the 2-xxct method and statistical significance was evaluated using the student's t-test
530			_aIssued also as CD
653		4	_aBuffalo
653		4	_aCattle
653		4	_aHeat stress
700	0		_aAbdelmohsen Hammam , _eSupervisor
700	0		_aAdel Khalil Ebrahim , _eSupervisor
700	0		_aMohamed Akmal Elghor , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c70454 _d70454