000			02846cam a2200349 a 4500
003			EG-GiCUC
005			20250223032219.0
008			190302s2018 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.11.07.M.Sc.2018.Sh.P
100	0		_aShimaa Ahmed Mosaad Basha
245	1	2	_aA pilot study of different morphological features in myeloproliferative neoplasms with calreticulin mutations / _cShimaa Ahmed Mosaad Basha ; Supervised Sherif Naseh Amin , Yasser Hassan Elnahass , Fatma Elrefaey
246	1	5	_aدراسة مبدئية صغيرة النطاق للصفات المورفولوجية المختلفة في الأورام الميلودية التكاثرية ذات الطفرة الجينية في الكالريتكولين
260			_aCairo : _bShimaa Ahmed Mosaad Basha , _c2018
300			_a109 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
520			_aBack ground Philadelphia chromosome{u2013}negative myeloproliferative neoplasms constitute a heterogeneous group of hematopoietic disorders manifested by a variety of morphologic changes in the bone marrow (BM).Calreticulin(CALR) is a multifunctional protein that binds Ca2+ions (a second messenger in signal transduction), rendering it inactive.The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal.CALR is located in storage compartments associated with the endoplasmic reticulum. Aim of the work: The aim of this study is to describe and correlate the morphologic features ofmyeloproliferative neoplasms(MPNs) especially ET and PMF with CALR mutations. Patients and Methods: The study was conducted on 68 MPNs patients;41/68 ET ,24/68 PMF and 3/68 post ET-MF.CALR Mutations By High ReslutionMelting(HRM) Analysis and Bone marrow trephine biopsy were done. Results : CALR-mut ET patients were more likely to show dense clustering of megakaryocytes compared to CALR-wt patients 8/10 (80%) vs 13/31 (41.9%) (p = 0.036).CALR-mut PMF showed megakaryocytes that more often exhibited nuclear hyperchromasia compared to CALR-wt PMF 3 / 4(75%) cases vs 11 / 20(55%) cases ( P = .0615). All post ET-MF patients showed variable megakaryocyte morphology. 3/4(75%) were JAK 2 positive .variable morphology included more abnormal bizzare ,hypolobated morphology
530			_aIssued also as CD
653		4	_aCALR
653		4	_aMPL
653		4	_aMPN
700	0		_aFatma Elrefaey , _eSupervisor
700	0		_aSherif Naseh Amin , _eSupervisor
700	0		_aYasser Hassan Elnahass , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c70590 _d70590