000 02440cam a2200325 a 4500
003 EG-GiCUC
008 190403s2018 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.10.10.M.Sc.2018.Ah.C
100 0 _aAhmed Mohammed Emam
245 1 0 _aComparative bacteriological and molecular studies on klebsiella species isolated from chicken and human /
_cAhmed Mohammed Emam ; Supervised Heidy Mohamed Shawky , Ahmed Samir Mohamed , Ebtesam Mohamed Mahmoud
246 1 5 _aدراسات بكتريولوجية وجزيئية مقارنة على أنواع الكليبسيللا المعزولة من الدجاج والانسان
260 _aCairo :
_bAhmed Mohammed Emam ,
_c2018
300 _a127 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520 _aIn the present study,1000 samples (liver, spleen, lung and trachea) from apparently healthy and diseased broiler, layer and baby chicks and 200 human samples bronchoalveolar lavage BAL, blood stream infection BSI, urine UR, wound W tissue T were collected to investigate the prevalence of Klebsiella spp. The isolation of Klebsiella from broilers revealed 4 isolates (5.8%), 8 isolates(5.3%) from layers, 3isolates from baby chicks (9.6%) and 16 human isolates (7.5%).Antimicrobial sensitivity testing against16 antibiotic agents revealed that all of isolates (100%) were sensitive to amikacin, Cefepime and imipenem followed by (97.3%) gentamicin, (94.6%) aztreonam and (86.5%) ciprofloxacin. While, (97.3%) of isolates were resistant to nalidixic acid followed by (94.6%) resistant to ampicillin, (83.8%) to tetracycline and (70.3%) to sulphamethoxazole/trimethoprim. Serological survey using; Molecular characterization of some ESBLs genes (blaTEM, blaCTX and blaSHV) using PCR showed that 6 isolates were positive for blaTEM gene and 5were positive for blaCTX gene. All isolates were negative for blaSHV gene
530 _aIssued also as CD
653 4 _aBacteriological
653 4 _aKlebsiella
653 4 _aMolecular
700 0 _aAhmed Samir Mohamed ,
_eSupervisor
700 0 _aEbtesam Mohamed Mahmoud ,
_eSupervisor
700 0 _aHeidy Mohamed Shawky ,
_eSupervisor
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c70650
_d70650