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| 003 | EG-GiCUC | ||
| 005 | 20250223032244.0 | ||
| 008 | 190413s2018 ua d f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.08.09.Ph.D.2018.Mo.P | ||
| 100 | 0 | _aMona Magdy Saber Moawad | |
| 245 | 1 | 0 |
_aPharmacological study of the combinatorial cytotoxic effect of certain drugs in human colon cancer cell lines / _cMona Magdy Saber Moawad ; Supervised Ezz Eldin Eldenshary , Samia A. Shouman , Noha Nagah Nassar |
| 246 | 1 | 5 | _aدراسة فارماكولوجية للتأثير السام لدمج بعض الادوية في خطوط خلايا القولون السرطانية في الانسان |
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_aCairo : _bMona Magdy Saber Moawad , _c2018 |
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_a180 P. : _bcharts ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - Faculty of Pharmacy - Department of Pharmacology and Toxicology | ||
| 520 | _aIntroduction: Colorectal cancer (CRC) remains a leading cause of death worldwide.The use of cisplatin as an anti-cancer drug in CRC is correlated with severe adverse effects and drug resistance. Utilizing combination of anticancer agents can effectively kill cancer through multiple pathways; nonetheless, improvements in their delivery are needed. Nano-cubosomes, thanks to the advantages of their liquid crystalline porous nano-architecture and capability for multi-drug encapsulation, appear to be of interest as nanocarriers for anti-cancer therapies. Hence, delivering cisplatin, as nano-cubosomes, concomitantly with other chemo-dugs can lead to a rationally designed therapy for chemo-resistant cancers and might overcome problems associated with conventional cisplatin treatment. Aim: The aim of the present study was to construct nano-cubosomes bearing cisplatin, metformin and cisplatin-metformin combination and investigate their effect on proliferation in Caco-2 and HCT-116 cell lines, as well as, tumorigenesis-associated metabolic markers in HCT-116 colorectal cancer cell line. Materials and methods: Nano-cubosomes bearing either cisplatin alone, metformin alone or cisplatin-metformin combination were formulated using emulsification technique. The loaded nano-cubosomes were characterized in vitro and the optimized formulations were selected.Their cytotoxic effects were investigated by Sulphorhodamine-B (SRB) assayin Caco-2 and HCT-116 cells. In HCT-116 cellsthe AMPK/mTOR metabolic pathwaywas analyzed using ELISA technique.Protein levels of AKT were determined using western blotting. Glucose, ATP, NADPH oxidase, LDH, glutathione (GSH) and caspase-3 activity were measured spectrophotometrically | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aCisplatin | |
| 653 | 4 | _aCRC | |
| 653 | 4 | _aMetformin | |
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_aEzz Eldin Eldenshary , _eSupervisor |
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| 700 | 0 |
_aNoha Nagah Nassar , _eSupervisor |
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| 700 | 0 |
_aSamia A. Shouman , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aShimaa _eCataloger |
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_2ddc _cTH |
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_c71428 _d71428 |
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