000			01896cam a2200325 a 4500
003			EG-GiCUC
008			190416s2018 ua d f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aPh.D
099			_aCai01.07.12.Ph.D.2018.As.I
100	0		_aAsmaa Elsayed Abdelhafez
245	1	0	_aIn vitro study on the propagation , conservation and DNA of Odontonema cuspidatum (Nees) O.Ktze Plant / _cAsmaa Elsayed Abdelhafez ; Supervised Salwa Salem Sakr , Mohamed Abdelkhalek Elkhateeb , Mamdouh Ahmed Elshamy
246	1	5	_aOdontonema cuspidatum دراسة معملية على الاكثار و الحفظ و الحمض النووى لنبات
260			_aCairo : _bAsmaa Elsayed Abdelhafez , _c2018
300			_a155 P. : _bcharts ; _c25cm
502			_aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Ornamental Horticulture
520			_aThis investigation was carried out during the period from 2014 to 2017. Using NaOCl at 2% for 25 min was the most effective. Culturing of the explants on full MS medium supplemented with 0.5 mg/l TDZ, was positively, 1mg/l IBA and 2 mg/l NAA resulted in the highest number of roots. peat moss+ sand at the ratio of 3:1 (v/v) to acclimatization. For encapsulation used sodium alginate (5 %) and 1.1 g/100ml CaCl2.2H2o2. Using sucrose at 50 g/l as osmotic agent was the best in all storage periods. RAPD- based DNA fingerprints gave no eridence of on variation during in vitro conservation
530			_aIssued also as CD
653		4	_aIn vitro
653		4	_aMicropropagation
653		4	_aTissue culture
700	0		_aMamdouh Ahmed Elshamy , _eSupervisor
700	0		_aMohamed Abdelkhalek Elkhateeb , _eSupervisor
700	0		_aSalwa Salem Sakr , _eSupervisor
905			_aAsmaa _eCataloger
905			_aNazla _eRevisor
942			_2ddc _cTH
999			_c71490 _d71490