000 02820cam a2200325 a 4500
003 EG-GiCUC
008 190418s2018 ua do f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aM.Sc
099 _aCai01.10.10.M.Sc.2018.Al.D
100 0 _aAlaa Mohamed Elsayed Elbadawi
245 1 0 _aDevelopment of nano-magnetic biosensor for rapid detection of resistant staphylococcus aureus from different sources /
_cAlaa Mohamed Elsayed Elbadawi ; Supervised Khaled F. ElAmry , Mahmoud D. Elhariri , Gihan M. Elmoghazy
246 1 5 _aتطوير جهاز استشعار بيولوجى نانو مغناطيسى للكشف السريع عن بكتيريا المكورات العنقودية الذهبية المقاومة للمضادات الحيوية من مصادر مختلفة
260 _aCairo :
_bAlaa Mohamed Elsayed Elbadawi ,
_c2018
300 _a236 P. :
_bcharts , photographs ;
_c25cm
502 _aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Micrbiology
520 _aThe emergence of antibiotic-resistant bacteria is a major concern in both human and veterinary medicine. In Egypt, Accurate numbers describing the prevalence of MRSA and VRSA are lacking especially in veterinary medicine. From this point of view, the aim of the study is to determine the occurrence of MRSA, VRSA isolated from different sources by both phenotypic and genotypic methods) and also to develop a rapid, accurate and sensitive technique for identification of resistant S.aureus. So, a total of 384 samples were collected from different sources as follow: 150 raw milk, 100 camel meat, 40 human nasal and wound swabs and 94 ration samples. 136(35.4%) isolates out of 384 were confirmed to be S.aureus by biochemical tests. They were tested against 9 antibiotic discs. There are three phenotypic methods for detection of MRSA: oxacillin disc (1æg),cefoxitin disc (30æg) and chromoginic agar(ORSAB).The most reliable method for detection of MRSA cefoxitin disc diffusion as identified in the present study and recorded by(CLSI,2016). So, the recovery rate of MRSA from different sources according to cefoxitin disc was 77 isolates distributed as follow: (44.6%)from milk,(86.4%) from camel,(70.9%)from human and (40.7%)from ration with the highest recovery rate recorded in Camel (86.4%). vancomycin disk does not differentiate vancomycin-susceptible, intermediate, and resistant isolates
530 _aIssued also as CD
653 4 _aMIC
653 4 _aMRSA
653 4 _aVRSA
700 0 _aGihan M. Elmoghazy ,
_eSupervisor
700 0 _aKhaled F. ElAmry ,
_eSupervisor
700 0 _aMahmoud D. Elhariri ,
_eSupervisor
905 _aAsmaa
_eCataloger
905 _aNazla
_eRevisor
942 _2ddc
_cTH
999 _c71542
_d71542