000			02109cam a2200313 a 4500
003			EG-GiCUC
008			190420s2018 ua do f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.12.05.M.Sc.2018.Mo.C
100	0		_aMohammad Mohammad Hady Rizk Alfola
245	1	0	_aComparative studies to evaluate different methods for ESBLs detection / _cMohammad Mohammad Hady Rizk Alfola ; Supervised Zenat Kamel , Mona Gamal Eldin Nada
246	1	5	_aدراسات مقارنة لتقييم الطرق المختلفة للكشف عن إنزيمات البتا لاكتاميزس ممتدة المفعول
260			_aCairo : _bMohammad Mohammad Hady Rizk Alfola , _c2018
300			_a76 P. : _bcharts , photographs ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany-Microbiology
520			_aIntroduction: Extended spectrum Ý-lactamases (ESBLs)-producing enterobacteriaceae are a clinical threat that may cause nosocomial as well as community acquitred infections. E.coli and klebsiella pneumoniae are among the most common gram negative bacilli causing urinary tract infections. This study investigated certain molecular characteristics of phenotypically proved ESBL-producing E. coli and K. pneumoniae. Materials and methods: This study includes a total of 64 community and hospital-acquired enterobacteriaceae suspected to produce ESBLs using routine antimicrobial susceptibility test. Identification of species of enterobacteriaceae was done by the API 20E identification system. ESBL production was detected by double disk synergy test (DDST) followed by detection of the encoding genes by PCR using primers for bla-TEM, bla-CTX-MI, bla-CTX-M2, bla-SHV and bla-PER genes
530			_aIssued also as CD
653		4	_aE. coli
653		4	_aESBLs
653		4	_aKlebsiella pneumoniae
700	0		_aMona Gamal Eldin Nada , _eSupervisor
700	0		_aZenat Kamel , _eSupervisor
905			_aNazla _eRevisor
905			_aSamia _eCataloger
942			_2ddc _cTH
999			_c71587 _d71587