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008 190424s2018 ua dh f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.09.14.Ph.D.2018.Sh.P
100 0 _aShaimaa Ali Hamouda
245 1 4 _aThe proliferation inhibiting and apoptosis inducing effects of chlorhexidine and essential oils mouthwashes on fibroblast cell line :
_bIn vitro study /
_cShaimaa Ali Hamouda ; Supervised Naglaa Elhossary , Nermine Raouf Amin
246 1 5 _aالتأثيرالمثبط للتكاثر والمحفزللموت المبرمج للكلورهيكسيدين والزيوت العطرية في خط الخلية الليفية :
_bدراسة معملية
260 _aCairo :
_bShaimaa Ali Hamouda ,
_c2018
300 _a112 P. :
_bcharts , facsimiles ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology
520 _aObjectives: This in vitro study evaluated the effect of Essential oils (EOs), Essential oils with sodium fluoride (EOF) and Chlorhexidine (CHX) mouth washes on viability and apoptosis of fibroblast cell line. Material and Methods: Baby hamster kidney -21 (BHK-21) fibroblast cell line was cultured and incubated in Eagle's Minimum Essential Medium (EMEM) containing, EOs, EOF and CHX with different doses and various exposure times. Control cells received an equal volume of EMEM without adding mouth rinses. Cell viability and apoptosis were evaluated using MTT assay and RT-PCR. Results: The EOs, EOF and CHX mouthwashes inhibited fibroblast cell viability in a dose and time dependant manner. Conclusions: CHX mouthwash was the most cytotoxic to fibroblasts as compared to EOs and EOF. Moreover, adding fluoride to EOs in low concentrations didn`t worsen the adverse effects of EOs on cell viability and apoptosis induction
530 _aIssued also as CD
653 4 _aChlorhexidine
653 4 _aEssential oils (EOs)
653 4 _aFibroblast cell line
700 0 _aNaglaa Elhossary ,
_eSupervisor
700 0 _aNermine Raouf Amin ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c71672
_d71672