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008 190509s2018 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.07.Ph.D.2018.As.S
100 0 _aAsmaa Mohamed Fteah Abdelaziz
245 1 0 _aStudy of MicroRNA 196a and 499 polymorphisms in hepatocellular carcinoma patients /
_cAsmaa Mohamed Fteah Abdelaziz ; Supervised Nehad Ahmed Mosaad , Mona Mohamed Hassan , Asmaa Ismail Ahmed
246 1 5 _aفى مرضى سرطان الكبد MICRORNA 196A and 499 دراسة تأثير التعددات الشكلية لـــ
260 _aCairo :
_bAsmaa Mohamed Fteah Abdelaziz ,
_c2018
300 _a170 P. :
_bcharts ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
520 _aBackground: Hepatocellular carcinoma (HCC) is the commonest primary tumor of the liver. MicroRNAs (miRNAs) can generate different functions for regulating RNA protein levels and balancing abnormalities. Single nucleotide polymorphisms (SNPs) in miRNAs were reported to increase patient susceptibility to cancer development and affect prognosis and survival. Objective: To evaluate MiRNA 196a2 (rs11614913) C>T and MiRNA 499 (rs3746444) A>G as potential biomarkers for HCV-related HCC development in Egyptian patients. Subjects and methods: A case-control study included 75 HCV-related HCC patients, 75 cirrhotic patients on top of HCV infection and 75 healthy controls. Genotyping of the candidate SNPs was performed by Real Time PCR. Liver and kidney function tests, hepatitis B and C markers and AFP were performed. Results: There was a significant difference in miRNA 499 (rs3746444) genotypes frequency between the three studied groups (p=0.009) and the combined microRNA 499 (AA+AG) genotypes were significantly higher in HCC cases than in cirrhosis and normal control groups (p=0.005). The frequency of the G allele was significantly lower in HCC cases than other groups (p=0.024) and significantly lower in HCC cases than normal control group (p=0.006) and [OR (95%CI) = 0.501 (0.304-0.825)]. Median AST, ALT, ALP, total bilirubin and direct bilirubin levels were found to be significantly lower in patients with GG genotype than those with (AA+AG) genotypes with (p = 0.002, 0.036, 0.006, 0.021 and 0.01) respectively. For miRNA 196a2 (rs11614913) C>T polymorphisms, no significant association was found with the risk for HCC development
530 _aIssued also as CD
653 4 _aGene polymorphism
653 4 _aHCC
653 4 _aMicroRNA 196a2 C>T
700 0 _aAsmaa Ismail Ahmed ,
_eSupervisor
700 0 _aMona Mohamed Hassan ,
_eSupervisor
700 0 _aNehad Ahmed Mosaad ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c71884
_d71884