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008 190509s2018 ua d f m 000 0 eng d
040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.07.Ph.D.2018.Do.M
100 0 _aDoaa Mamdouh Aly Ahmad Abubakr
245 1 0 _aMicroRNA let-7a-1, let-7d and let-7f gene expression in hepatocellular carcinoma patients /
_cDoaa Mamdouh Aly Ahmad Abu-Bakr ; Supervised Nadida Abdulhameed Gohar , Afaf Ahmed Abdelhady , Mona Mohsen Abdulsalam
246 1 5 _aفى مرضى سرطان الكبد let-7fو let-7d و let-7a-1 التعبير الجينى الخاص بالأحماض الريبونيوكليك الدقيقة
260 _aCairo :
_bDoaa Mamdouh Aly Ahmad Abubakr ,
_c2018
300 _a228 P. :
_bcharts ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Clinical and Chemical Pathology
520 _aBackground: Hepatocellular carcinoma is one of the most common and lethal malignant tumors worldwide. MicroRNAs are involved in the epigenetic mechanism{u2019}s implicated in HCC molecular pathogenesis. Measuring the levels of the circulating miRNAs to be used as biomarkers for malignancies has recently been investigated in the hope of identifying unique miRNA signatures in human tumors. Two clusters of microRNAs have been selected in this study; Let7-a-1, let-7d-1, let-7f-1 cluster and miR143/145 cluster. Aim of the study: To identify miRNA let-7 a-1, d-1, f-1 cluster and miRNA143/ 145 cluster gene expressions in HCC patients. Methods: The study included 40 Hepatocellular carcinoma patients, 40 chronic HCV patients divided into 2 subgroups, 20 patients with liver cirrhosis and 20 patients without liver cirrhosis, and 40 apparently healthy subjects as a control group. They were subjected to routine laboratory investigations and gene expression analysis for miRNA let-7 a-1, d-1, f-1 cluster and miRNA 143/ 145 cluster using quantitative SYBR Green reverse-transcription Real-Time polymerase chain reaction
530 _aIssued also as CD
653 4 _aHepatocellular carcinoma
653 4 _aLet-7d-1 miRNA
653 4 _aMicroRNA - Let-7a-1 miRNA
700 0 _aAfaf Ahmed Abdelhady ,
_eSupervisor
700 0 _aMona Mohsen Abdulsalam ,
_eSupervisor
700 0 _aNadida Abdulhameed Gohar ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aSamia
_eCataloger
942 _2ddc
_cTH
999 _c71898
_d71898