| 000 | 02903cam a2200349 a 4500 | ||
|---|---|---|---|
| 003 | EG-GiCUC | ||
| 005 | 20250223032313.0 | ||
| 008 | 190613s2017 ua dh f m 000 0 eng d | ||
| 040 |
_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aM.Sc | ||
| 099 | _aCai01.09.12.M.Sc.2017.He.P | ||
| 100 | 0 | _aHeba Shawky Helmi Abdelhamid | |
| 245 | 1 | 4 |
_aThe proliferation effect of platelet-rich plasma on human dental pulp stem cells : _bIn vitro study / _cHeba Shawky Helmi Abdelhamid ; Supervised Mohamad Gad Abas , Ahmed Wael Abouzeid , Dina Sabry Abdelfattah |
| 246 | 1 | 5 | _aتأثير البلازما الغنية بالصفائح الدموية على تكاثر الخلايا الجذعية المستخرجة من لب الأسنان البشرية |
| 260 |
_aCairo : _bHeba Shawky Helmi Abdelhamid , _c2017 |
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| 300 |
_a127 P. : _bcharts , facsimiles ; _c25cm |
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| 502 | _aThesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral Biology | ||
| 520 | _aBackground: Dental pulp stem cells (DPSCs) are {u200E}considered an easily accessible source of mesenchymal {u200E}stem cells holding great promise for use in tissue repair and {u200E}regenerative medicine. In order for DPSCs to be used in {u200E}therapeutic clinical applications, issues like safely {u200E}enhancing culture expansion need to be addressed. {u200E}Objective: In this research, we aimed to assess the safety of {u200E}platelet rich plasma (PRP) as a promoter of proliferation in {u200E}comparison to routinely used animal derived supplements. {u200E}Methods: The effect of PRP on the proliferation of DPSCs {u200E}was assessed by MTT assay. Expression of stemness-{u200E}related genes OCT4 & INTGRIN1 was analyzed by real-{u200E}time quantitative PCR. DNA sequencing was performed {u200E}for OCT4 & INTGRIN1 genes to ensure that the isolated {u200E}DPSCs stem cell properties were not altered by the PRP {u200E}supplemented media. Results: At a concentration of 1% {u200E}with platelet count of 1.5 x 106/cm3, PRP was able to {u200E}significantly increase the proliferation rate while {u200E}maintaining the viability of DPSCs in comparison to {u200E}routinely used 15% FBS. Mesenchymal stem cell surface {u200E}markers expression (CD29, CD105) were not altered by {u200E}PRP supplementation. Moreover, PRP in cultures {u200E}significantly promoted expression of stemness markers {u200E}OCT4 & INTGRIN1 compared with 10% FBS | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aDental pulp stem cells | |
| 653 | 4 | _aPlatelet rich plasma | |
| 653 | 4 | _aProliferation | |
| 700 | 0 |
_aAhmed Wael Abouzeid , _eSupervisor |
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| 700 | 0 |
_aDina Sabry Abdelfattah , _eSupervisor |
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| 700 | 0 |
_aMohamad Gad Abas , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
| 905 |
_aNazla _eRevisor |
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| 905 |
_aShimaa _eCataloger |
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| 942 |
_2ddc _cTH |
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| 999 |
_c72368 _d72368 |
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