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040 _aEG-GiCUC
_beng
_cEG-GiCUC
041 0 _aeng
049 _aDeposite
097 _aPh.D
099 _aCai01.11.19.Ph.D.2018.Ah.M
100 0 _aAhmed Salah Emira Aly
245 1 0 _aMultiplex PCR for detection of five selected carbapenemase genes in comparative study with modified-hodge test in gram-negative bacilli isolated from healthcare-associated infections among critically Ill patients /
_cAhmed Salah Emira Aly ; Supervised Abdelfattah Mohamed Attia , Reham Ali Dwedar , Nazmy Edward Seif
246 1 5 _aتفاعل البلمرة المتسلسل المتعدد للكشف عن خمسة جينات للكاربابينيماز في دراسة مقارنة مع اختبار هودج المعدّل في البكتيريا سالبة الجرام المعزولة من حالات العدوى المرتبطة بالرعاية الصحية بين مرضى الحالات الحرجة
260 _aCairo :
_bAhmed Salah Emira Aly ,
_c2018
300 _a118 P. :
_bcharts ;
_c25cm
502 _aThesis (Ph.D.) - Cairo University - Faculty of Medicine - Department of Microbiology and Immunology
520 _aBackground:Carbapenemase producing Gram-negative bacilli have been reported to be an important cause of hospital associated infections (HAIs), especially in critically ill patients with device associated infections (DAIs). Aim of the work: To estimate the extent of carbapenemase producing Gram-negative bacteria in critically ill patients from Kasr El-Aini, Cairo University hospitals and to compare the Modified-Hodge test (MHT) as a phenotypic confirmatory test with the PCR as a gold standard genotypic method for their diagnosis. Patients and methods:95 bacterial isolates from 75 critically ill patients were collected over a period of six months from November 2016 to April 2017 from Cairo university hospitals. These isolates were subjected to susceptibility testing for carbapenems as per CLSI guidelines and were further phenotypically screened for the production of carbapenemase by MHT, then were subjected to genotypic analysis by multiplex PCR
530 _aIssued also as CD
653 4 _aCarbapenemase
653 4 _aModified Hodge test
653 4 _aSilent Genes
700 0 _aAbdelfattah Mohamed Attia ,
_eSupervisor
700 0 _aNazmy Edward Seif ,
_eSupervisor
700 0 _aReham Ali Dwedar ,
_eSupervisor
856 _uhttp://172.23.153.220/th.pdf
905 _aNazla
_eRevisor
905 _aShimaa
_eCataloger
942 _2ddc
_cTH
999 _c72764
_d72764