000			03131cam a2200349 a 4500
003			EG-GiCUC
005			20250223032357.0
008			190914s2019 ua dh f m 000 0 eng d
040			_aEG-GiCUC _beng _cEG-GiCUC
041	0		_aeng
049			_aDeposite
097			_aM.Sc
099			_aCai01.10.10.M.Sc.2019.Ab.M
100	0		_aAbdoulrazak Omar Ali
245	1	0	_aMolecular recognition of brucella based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry and molecular imprinted polymers / _cAbdoulrazak Omar Ali ; Supervised Wagih A. Gad Elsaid , Mahmoud D. Elhariri , Ashraf E. M. Sayour
246	1	5	_aتعرف جزيئي على البروسيلا قائم على مطياف الكتلة المعتمد على زمن طيران الببتيدات المتأينة بالليزر الممتز بمساعدة وسط مح{u٠٦أأ}ط والمبلمرات المطبوعة جزيئيا
260			_aCairo : _bAbdoulrazak Omar Ali , _c2019
300			_a120 P. : _bcharts , facsimiles ; _c25cm
502			_aThesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
520			_aBrucella is an expanding genus of Gram-negative intracellular wide host ranging pathogens. This work aimed at investigating molecular recognition of Brucella by MALDI-TOF MS proteomic fingerprinting as well as novel plastic antibodies by developing and characterizing molecularly-imprinted hydrogels to grasp all surface epitopes at one go for whole cell recognition of Brucellaabortus, B. melitensisand B. suis known to exist among livestock in Egypt. An MSP library of 11 reference Brucella strains was created. A dendrogram for reference strains was plotted to analyze phyloproteomic relations. Based on bacteriologic and proteomic biotyping of 45 field isolates, a map revealed the geographic distribution of Brucellamelitensis and B. abortus from 69 unvaccinated seropositive ruminants in 12 governorates during 2015.The MALDI-TOF MS was re-evaluated as a revolutionary molecular tool for Brucella identification reviewing the pros and cons of the technique suggesting recent methods to tackle existing hitches. E{uFB00}ective bacterial recognition using a cell-imprinted polymer (CIP) formed on a 96-well microplate was achieved within 30 minutes.The polymer could discriminate the target strain from other strains with high selectivity reaching approximately 20 folds. It was concluded that bacteriologic and MALDI results fully matched thanks to the limited diversity of Brucella isolates and the narrow MSP library. The CIP approach proved valuable for rapid direct Brucella recognition and quantification colorimetrically in microtiter plates after full validation
530			_aIssued also as CD
653		4	_aBrucella
653		4	_aHydrogel
653		4	_aMIPS
700	0		_aAshraf E. M. Sayour , _eSupervisor
700	0		_aMahmoud D. Elhariri , _eSupervisor
700	0		_aWagih A. Gad Elsaid , _eSupervisor
856			_uhttp://172.23.153.220/th.pdf
905			_aNazla _eRevisor
905			_aShimaa _eCataloger
942			_2ddc _cTH
999			_c73859 _d73859