| 000 | 03016cam a2200349 a 4500 | ||
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| 003 | EG-GiCUC | ||
| 005 | 20250223032411.0 | ||
| 008 | 191003s2019 ua h f m 000 0 eng d | ||
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_aEG-GiCUC _beng _cEG-GiCUC |
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| 041 | 0 | _aeng | |
| 049 | _aDeposite | ||
| 097 | _aPh.D | ||
| 099 | _aCai01.24.05.Ph.D.2019.He.E | ||
| 100 | 0 | _aHeba Elsayed Mahfouz Elzorkany | |
| 245 | 1 | 0 |
_aEfficiency of biocompatible quantum dots for cellular imaging using confocal laser scanning microscope / _cHeba Elsayed Mahfouz Elzorkany ; Supervised Tareq Youssef , Salwa Thabet , Taher A. Salah Eldin |
| 246 | 1 | 5 | _aكفاءة نقاط الكم الملائمة حيويا للتصوير باستخدام الميكروسكوب الليزرى الماسح البؤرى |
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_aCairo : _bHeba Elsayed Mahfouz Elzorkany , _c2019 |
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_a180 P. : _bfacsimiles ; _c25cm |
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| 502 | _aThesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Science - Department of Laser Application in Environmental Metrology Photochemistry and Agriculture | ||
| 520 | _aQuantum dots (QDs) are zero-dimensional systems wherein charge carriers (i.e., electrons and holes) are confined in all three dimensions. QDs are a very interesting nanomaterial with unique characteristics, which could help in many clinical and pharmaceutical purposes. In this study, we aimed to investigate the efficiency of silica-coated CdSe/ZnS (CdSe/ZnS-SiO NCs) and Graphene 2 nanocrystals (GQDs) for imaging purposes. CdSe quantum dots (QDs) were synthesized by organometallic routes and were coated with ZnS shell by injecting solutions of diethylzinc (Zn (Et) ₂) and hexamethyldislathiane ((TMS)₂ S) as precursors for zinc and sulfur ions respectively. On the other hand, GQDs were synthesized via glucose pyrolysis. Then, the prepared NCs overcoated with silica using tetraethyl orthosilicate (TEOS) as a silica precursor. QDs were characterized by UV-Vis absorption, emission spectroscopy TEM, XRD, and DLS. The biocompatibility of silica-coated QDs was tested by evaluating mitochondrial activity and alkaline comet assay of liver hepatocellular carcinoma (HepG2) cells exposed to different concentrations of QDs. The intracellular uptake and localization of QDs in HepG2 cells, Bacillus subtilis (B. subtilis) and chlamydomonas reinhardtii (C. reinhardtii) were monitored by fluorescence imaging using confocal laser scanning microscopy (CLSM) up to eight hours. Results showed that silica coating yielded final particles{u2019} size around 30 nm possessing strong luminescence property | ||
| 530 | _aIssued also as CD | ||
| 653 | 4 | _aBiocompatible quantum dots | |
| 653 | 4 | _aConfocal laser scanning microscope | |
| 653 | 4 | _aQuantum dots (QDs) | |
| 700 | 0 |
_aSalwa Thabet , _eSupervisor |
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| 700 | 0 |
_aTaher Salah Eldin , _eSupervisor |
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| 700 | 0 |
_aTareq Youssef ِAbdelwahed , _eSupervisor |
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| 856 | _uhttp://172.23.153.220/th.pdf | ||
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_aNazla _eRevisor |
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_aSamia _eCataloger |
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_2ddc _cTH |
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_c74283 _d74283 |
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